Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator.

Isabelle Colson; Edgar Hartsuiker

doi:10.1101/pdb.prot091967

Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator.

Isabelle Colson
, Edgar Hartsuiker

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.

Iaith wreiddiol	Saesneg
Cyfnodolyn	Cold Spring Harbor Protocols
Cyfrol	2018
Rhif cyhoeddi	2
Dyddiad ar-lein cynnar	21 Gorff 2017
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1101/pdb.prot091967
Statws	Cyhoeddwyd - Chwef 2018

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10.1101/pdb.prot091967

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@article{3b7603106ebf45c2b80d167ede19745c,

title = "Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator.",

abstract = "n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed. ",

author = "Isabelle Colson and Edgar Hartsuiker",

year = "2018",

month = feb,

doi = "10.1101/pdb.prot091967",

language = "English",

volume = "2018",

journal = "Cold Spring Harbor Protocols",

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publisher = "Cold Spring Harbor Laboratory Press",

number = "2",

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AU - Colson, Isabelle

AU - Hartsuiker, Edgar

PY - 2018/2

Y1 - 2018/2

N2 - n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.

AB - n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.

U2 - 10.1101/pdb.prot091967

DO - 10.1101/pdb.prot091967

M3 - Article

SN - 1559-6095

VL - 2018

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 2

ER -

Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator.

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Mynediad at Ddogfen

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