TY - CHAP
T1 - Prenylated FMN: biosynthesis, purification, and Fdc1 activation
AU - Khusnutdinova, Anna N.
AU - Xiao, Johnny
AU - Wang, Po-Hsiang
AU - Batyrova, Khorcheska
AU - Flick, Robert
AU - Edwards, Elizabeth
AU - Yakunin, Alexander
PY - 2019
Y1 - 2019
N2 - Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.
AB - Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.
U2 - 10.1016/bs.mie.2019.03.021
DO - 10.1016/bs.mie.2019.03.021
M3 - Chapter
VL - 620
T3 - Methods in Enzymology
SP - 469
EP - 488
BT - New Approaches for Flavin Catalysis
A2 - Palfey, Bruce A.
PB - Elsevier
ER -