Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes

Julia S Sabirova; Anke Becker; Heinrich Lünsdorf; Jean-Marc Nicaud; Kenneth N Timmis; Peter N Golyshin

doi:10.1111/j.1574-6968.2011.02279.x

Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes

Julia S Sabirova
, Anke Becker
, Heinrich Lünsdorf
, Jean-Marc Nicaud
, Kenneth N Timmis
, Peter N Golyshin

Department of Experimental Clinical and Health Psychology, Ghent University, Ghent, Belgium

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	160-8
Nifer y tudalennau	9
Cyfnodolyn	FEMS Microbiology Letters
Cyfrol	319
Rhif cyhoeddi	2
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1111/j.1574-6968.2011.02279.x
Statws	Cyhoeddwyd - Meh 2011

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10.1111/j.1574-6968.2011.02279.x

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@article{06f5d75531cc498eb564152b5d2dc57f,

title = "Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes",

abstract = "The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.",

keywords = "Alcanivoraceae/genetics, Alkanes/metabolism, Bacterial Proteins/genetics, Biodegradation, Environmental, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Seawater/microbiology, Transcription, Genetic",

author = "Sabirova, \{Julia S\} and Anke Becker and Heinrich L{\"u}nsdorf and Jean-Marc Nicaud and Timmis, \{Kenneth N\} and Golyshin, \{Peter N\}",

year = "2011",

month = jun,

doi = "10.1111/j.1574-6968.2011.02279.x",

language = "English",

volume = "319",

pages = "160--8",

journal = "FEMS Microbiology Letters",

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publisher = "Oxford University Press",

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AU - Sabirova, Julia S

AU - Becker, Anke

AU - Lünsdorf, Heinrich

AU - Nicaud, Jean-Marc

AU - Timmis, Kenneth N

AU - Golyshin, Peter N

PY - 2011/6

Y1 - 2011/6

N2 - The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.

AB - The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.

KW - Alcanivoraceae/genetics

KW - Alkanes/metabolism

KW - Bacterial Proteins/genetics

KW - Biodegradation, Environmental

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Bacterial

KW - Molecular Sequence Data

KW - Seawater/microbiology

KW - Transcription, Genetic

U2 - 10.1111/j.1574-6968.2011.02279.x

DO - 10.1111/j.1574-6968.2011.02279.x

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JO - FEMS Microbiology Letters

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Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes

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