Detection of covalent DNA-bound Spo11 and topoisomerase complexes

E. Hartsuiker; H. Tsubouchi

doi:10.1007/978-1-61779-129-1_5

Detection of covalent DNA-bound Spo11 and topoisomerase complexes

E. Hartsuiker
, H. Tsubouchi (Editor)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA

Original language	English
Title of host publication	DNA recombination: Methods and Protocols
Publisher	Springer Science and Business Media LLC
Pages	65-77
ISBN (Print)	9781617791291
DOIs	https://doi.org/10.1007/978-1-61779-129-1_5
Publication status	Published - 28 Apr 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1007/978-1-61779-129-1_5

Cite this

@inbook{978e31e10bdd4b19adfcb418fd43a530,

title = "Detection of covalent DNA-bound Spo11 and topoisomerase complexes",

abstract = "Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA",

author = "E. Hartsuiker and H. Tsubouchi",

year = "2011",

month = apr,

day = "28",

doi = "10.1007/978-1-61779-129-1\_5",

language = "English",

isbn = "9781617791291",

pages = "65--77",

booktitle = "DNA recombination: Methods and Protocols",

publisher = "Springer Science and Business Media LLC",

}

TY - CHAP

T1 - Detection of covalent DNA-bound Spo11 and topoisomerase complexes

AU - Hartsuiker, E.

A2 - Tsubouchi, H.

PY - 2011/4/28

Y1 - 2011/4/28

N2 - Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA

AB - Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA

U2 - 10.1007/978-1-61779-129-1_5

DO - 10.1007/978-1-61779-129-1_5

M3 - Chapter

SN - 9781617791291

SP - 65

EP - 77

BT - DNA recombination: Methods and Protocols

PB - Springer Science and Business Media LLC

ER -

Detection of covalent DNA-bound Spo11 and topoisomerase complexes

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this