Determination of poly-β-hydroxyalkanoate in Peat

David Dowrick; Fatimah Yusoff; Rachel Gough; Christopher Freeman

doi:10.1080/00103624.2017.1282506

Determination of poly-β-hydroxyalkanoate in Peat

David Dowrick, Fatimah Yusoff, Rachel Gough, Christopher Freeman

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Abstract

Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.

Original language	English
Pages (from-to)	576-580
Journal	Communications in Soil Science and Plant Analysis
Volume	48
Issue number	5
Early online date	6 Feb 2017
DOIs	https://doi.org/10.1080/00103624.2017.1282506
Publication status	Published - Feb 2017

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title = "Determination of poly-β-hydroxyalkanoate in Peat",

abstract = "Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change. ",

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AU - Yusoff, Fatimah

AU - Gough, Rachel

AU - Freeman, Christopher

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N2 - Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.

AB - Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.

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DO - 10.1080/00103624.2017.1282506

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JO - Communications in Soil Science and Plant Analysis

JF - Communications in Soil Science and Plant Analysis

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ER -

Determination of poly-β-hydroxyalkanoate in Peat

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