Double-stranded library preparation for ancient and other degraded samples

Kristin Henneberger; Axel Barlow; Johanna Paijmans

doi:10.1007/978-1-4939-9176-1_8

Double-stranded library preparation for ancient and other degraded samples

Kristin Henneberger, Axel Barlow, Johanna Paijmans

University of Potsdam

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

Original language	English
Title of host publication	Ancient DNA: methods and protocols. Second Edition
Publisher	Humana New York, NY
Pages	65-73
ISBN (Electronic)	9781493991761
ISBN (Print)	9781493991754
DOIs	https://doi.org/10.1007/978-1-4939-9176-1_8
Publication status	Published - 16 Mar 2019
Externally published	Yes

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10.1007/978-1-4939-9176-1_8

Cite this

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title = "Double-stranded library preparation for ancient and other degraded samples",

abstract = "High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.",

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AB - High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

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BT - Ancient DNA: methods and protocols. Second Edition

PB - Humana New York, NY

ER -

Double-stranded library preparation for ancient and other degraded samples

Abstract

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