Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA

Alexandre B. de Menezes; James E McDonald; Heather E. Allison; Alan J.  McCarthy

doi:10.1128/AEM.07314-11

Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA

Alexandre B. de Menezes, James E McDonald, Heather E. Allison, Alan J. McCarthy

University of Liverpool

Research output: Contribution to journal › Article › peer-review

Abstract

The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.

Original language	English
Pages (from-to)	3495-9
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	9
DOIs	https://doi.org/10.1128/AEM.07314-11
Publication status	Published - May 2012

Keywords

Bacterial Load
Cellulose
DNA, Bacterial
DNA, Ribosomal
Fresh Water
Geologic Sediments
Micromonospora
Molecular Sequence Data
RNA, Ribosomal, 16S
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Journal Article

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10.1128/AEM.07314-11

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@article{6723dc253471420fbc00f4d2ea02948c,

title = "Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA",

abstract = "The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.",

keywords = "Bacterial Load, Cellulose, DNA, Bacterial, DNA, Ribosomal, Fresh Water, Geologic Sediments, Micromonospora, Molecular Sequence Data, RNA, Ribosomal, 16S, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Journal Article",

author = "{de Menezes}, {Alexandre B.} and McDonald, {James E} and Allison, {Heather E.} and McCarthy, {Alan J.}",

year = "2012",

month = may,

doi = "10.1128/AEM.07314-11",

language = "English",

volume = "78",

pages = "3495--9",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "9",

}

Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA. / de Menezes, Alexandre B.; McDonald, James E; Allison, Heather E. et al.
In: Applied and Environmental Microbiology, Vol. 78, No. 9, 05.2012, p. 3495-9.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA

AU - de Menezes, Alexandre B.

AU - McDonald, James E

AU - Allison, Heather E.

AU - McCarthy, Alan J.

PY - 2012/5

Y1 - 2012/5

N2 - The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.

AB - The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.

KW - Bacterial Load

KW - Cellulose

KW - DNA, Bacterial

KW - DNA, Ribosomal

KW - Fresh Water

KW - Geologic Sediments

KW - Micromonospora

KW - Molecular Sequence Data

KW - RNA, Ribosomal, 16S

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Analysis, DNA

KW - Journal Article

U2 - 10.1128/AEM.07314-11

DO - 10.1128/AEM.07314-11

M3 - Article

C2 - 22389367

SN - 0099-2240

VL - 78

SP - 3495

EP - 3499

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 9

ER -

Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA

Abstract

Keywords

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