Isolation of new Klebsiella pneumoniae phage PSKP16

Sara Sadeqi; Slawomir Michniewski; Farhad Nikkhahi; Eleanor Jameson; Seyed Mahmoud Amin Marashi

doi:10.18502/ijm.v15i1.11919

Isolation of new Klebsiella pneumoniae phage PSKP16

Sara Sadeqi, Slawomir Michniewski, Farhad Nikkhahi, Eleanor Jameson, Seyed Mahmoud Amin Marashi

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.

MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.

RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.

CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.

Original language	English
Pages (from-to)	62-68
Number of pages	7
Journal	Iranian journal of microbiology
Volume	15
Issue number	1
DOIs	https://doi.org/10.18502/ijm.v15i1.11919
Publication status	Published - 1 Feb 2023

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title = "Isolation of new Klebsiella pneumoniae phage PSKP16",

abstract = "BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.",

author = "Sara Sadeqi and Slawomir Michniewski and Farhad Nikkhahi and Eleanor Jameson and Marashi, {Seyed Mahmoud Amin}",

note = "Copyright {\textcopyright} 2023 The Authors. Published by Tehran University of Medical Sciences.",

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doi = "10.18502/ijm.v15i1.11919",

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AU - Sadeqi, Sara

AU - Michniewski, Slawomir

AU - Nikkhahi, Farhad

AU - Jameson, Eleanor

AU - Marashi, Seyed Mahmoud Amin

PY - 2023/2/1

Y1 - 2023/2/1

N2 - BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.

AB - BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.

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JO - Iranian journal of microbiology

JF - Iranian journal of microbiology

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Isolation of new Klebsiella pneumoniae phage PSKP16

Abstract

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