TY - JOUR
T1 - On the effects of 30.5 GHz sinusoidal wave exposure on glioblastoma organoids
AU - Rampazzo, Elena
AU - Persano, Luca
AU - Karim, Nissar
AU - Hodgking, George
AU - Pinto, Rosanna
AU - Casciati, Arianna
AU - Tanori, Mirella
AU - Zambotti, Alessandro
AU - Bresolin, Silvia
AU - Cani, Alice
AU - Pannicelli, Alessandro
AU - Davies, Ilan W
AU - Hancock, Cristopher
AU - Palego, Cristiano
AU - Viola, Giampietro
AU - Mancuso, Mariateresa
AU - Merla, Caterina
PY - 2024/5/31
Y1 - 2024/5/31
N2 - Glioblastoma (grade IV) is the most aggressive primary brain tumor in adults, representing one of the biggest therapeutic challenges due to its highly aggressive nature. In this study, we investigated the impact of millimeter waves on tridimensional glioblastoma organoids derived directly from patient tumors. Our goal was to explore novel therapeutic possibilities in the fight against this challenging disease. The exposure setup was meticulously developed in-house, and we employed a comprehensive dosimetry approach, combining numerical and experimental methods. Biological endpoints included a global transcriptional profiling analysis to highlight possible deregulated pathways, analysis of cell morphological changes, and cell phenotypic characterization which are all important players in the control of glioblastoma progression. Our results revealed a significant effect of continuous millimeter waves at 30.5 GHz on cell proliferation and apoptosis, although without affecting the differentiation status of glioblastoma cells composing the organoids. Excitingly, when applying a power level of 0.1 W (Root Mean Square), we discovered a remarkable (statistically significant) therapeutic effect when combined with the chemotherapeutic agent Temozolomide, leading to increased glioblastoma cell death. These findings present a promising interventional window for treating glioblastoma cells, harnessing the potential therapeutic benefits of 30.5 GHz CW exposure. Temperature increase during treatments was carefully monitored and simulated with a good agreement, demonstrating a negligible involvement of the temperature elevation for the observed effects. By exploring this innovative approach, we pave the way for improved future treatments of glioblastoma that has remained exceptionally challenging until now. [Abstract copyright: Copyright © 2024 Rampazzo, Persano, Karim, Hodgking, Pinto, Casciati, Tanori, Zambotti, Bresolin, Cani, Pannicelli, Davies, Hancock, Palego, Viola, Mancuso and Merla.]
AB - Glioblastoma (grade IV) is the most aggressive primary brain tumor in adults, representing one of the biggest therapeutic challenges due to its highly aggressive nature. In this study, we investigated the impact of millimeter waves on tridimensional glioblastoma organoids derived directly from patient tumors. Our goal was to explore novel therapeutic possibilities in the fight against this challenging disease. The exposure setup was meticulously developed in-house, and we employed a comprehensive dosimetry approach, combining numerical and experimental methods. Biological endpoints included a global transcriptional profiling analysis to highlight possible deregulated pathways, analysis of cell morphological changes, and cell phenotypic characterization which are all important players in the control of glioblastoma progression. Our results revealed a significant effect of continuous millimeter waves at 30.5 GHz on cell proliferation and apoptosis, although without affecting the differentiation status of glioblastoma cells composing the organoids. Excitingly, when applying a power level of 0.1 W (Root Mean Square), we discovered a remarkable (statistically significant) therapeutic effect when combined with the chemotherapeutic agent Temozolomide, leading to increased glioblastoma cell death. These findings present a promising interventional window for treating glioblastoma cells, harnessing the potential therapeutic benefits of 30.5 GHz CW exposure. Temperature increase during treatments was carefully monitored and simulated with a good agreement, demonstrating a negligible involvement of the temperature elevation for the observed effects. By exploring this innovative approach, we pave the way for improved future treatments of glioblastoma that has remained exceptionally challenging until now. [Abstract copyright: Copyright © 2024 Rampazzo, Persano, Karim, Hodgking, Pinto, Casciati, Tanori, Zambotti, Bresolin, Cani, Pannicelli, Davies, Hancock, Palego, Viola, Mancuso and Merla.]
KW - numerical and experimental dosimetry
KW - combined treatments
KW - glioblastoma organoids
KW - millimeter waves
KW - transcriptomics
U2 - 10.3389/fonc.2024.1307516
DO - 10.3389/fonc.2024.1307516
M3 - Article
C2 - 38884089
SN - 2234-943X
VL - 14
JO - Frontiers in Oncology
JF - Frontiers in Oncology
M1 - 1307516
ER -