The anti-microbial properties of slate particles used in a traditional balm and the effect on the growth of Staphylococcus aureus

Paul Baker; Adam Charlton; I. Jones

The anti-microbial properties of slate particles used in a traditional balm and the effect on the growth of Staphylococcus aureus

Paul Baker
, Adam Charlton
, I. Jones

BioComposites

Mon Naturals Ltd.

Research output: Contribution to journal › Article › peer-review

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Abstract

Objective

The study investigated the growth S. aureus on slate particles that are an important component in a traditional balm used in the treatment of sores and burns. S. aureus is one of a variety of opportunistic pathogens arising from burns.

Methods

The initial experiments were performed by culturing S. aureus in dilute liquid medium with slate particles using horizontal shaking at 300 rpm. It was considered that the results may have arisen due to an artefact of the experiment and culturing was performed differently to address potential problems. Therefore, culturing was performed using vertical end-over-end rotation at much lower speeds of 40 rpm.

Different culturing strategies were also explored using spread plating and microplate MPN dilutions that were monitored using a microplate reader. In addition, extractives were recovered from the slate particles and these were assessed for anti-microbial activity.

Original language	English
Journal	Journal of Traditional and Complementary Medicine
Publication status	Unpublished - 1 Sept 2020

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antimicrobial properties of slateAccepted author manuscript, 211 KBLicence: CC BY-NC-ND

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title = "The anti-microbial properties of slate particles used in a traditional balm and the effect on the growth of Staphylococcus aureus",

abstract = "Objective The study investigated the growth S. aureus on slate particles that are an important component in a traditional balm used in the treatment of sores and burns. S. aureus is one of a variety of opportunistic pathogens arising from burns. Methods The initial experiments were performed by culturing S. aureus in dilute liquid medium with slate particles using horizontal shaking at 300 rpm. It was considered that the results may have arisen due to an artefact of the experiment and culturing was performed differently to address potential problems. Therefore, culturing was performed using vertical end-over-end rotation at much lower speeds of 40 rpm. Different culturing strategies were also explored using spread plating and microplate MPN dilutions that were monitored using a microplate reader. In addition, extractives were recovered from the slate particles and these were assessed for anti-microbial activity.",

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journal = "Journal of Traditional and Complementary Medicine",

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T1 - The anti-microbial properties of slate particles used in a traditional balm and the effect on the growth of Staphylococcus aureus

AU - Baker, Paul

AU - Charlton, Adam

AU - Jones, I.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Objective The study investigated the growth S. aureus on slate particles that are an important component in a traditional balm used in the treatment of sores and burns. S. aureus is one of a variety of opportunistic pathogens arising from burns. Methods The initial experiments were performed by culturing S. aureus in dilute liquid medium with slate particles using horizontal shaking at 300 rpm. It was considered that the results may have arisen due to an artefact of the experiment and culturing was performed differently to address potential problems. Therefore, culturing was performed using vertical end-over-end rotation at much lower speeds of 40 rpm. Different culturing strategies were also explored using spread plating and microplate MPN dilutions that were monitored using a microplate reader. In addition, extractives were recovered from the slate particles and these were assessed for anti-microbial activity.

AB - Objective The study investigated the growth S. aureus on slate particles that are an important component in a traditional balm used in the treatment of sores and burns. S. aureus is one of a variety of opportunistic pathogens arising from burns. Methods The initial experiments were performed by culturing S. aureus in dilute liquid medium with slate particles using horizontal shaking at 300 rpm. It was considered that the results may have arisen due to an artefact of the experiment and culturing was performed differently to address potential problems. Therefore, culturing was performed using vertical end-over-end rotation at much lower speeds of 40 rpm. Different culturing strategies were also explored using spread plating and microplate MPN dilutions that were monitored using a microplate reader. In addition, extractives were recovered from the slate particles and these were assessed for anti-microbial activity.

M3 - Article

SN - 2225-4110

JO - Journal of Traditional and Complementary Medicine

JF - Journal of Traditional and Complementary Medicine

ER -

The anti-microbial properties of slate particles used in a traditional balm and the effect on the growth of Staphylococcus aureus

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