Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes

Julia S Sabirova; Anke Becker; Heinrich Lünsdorf; Jean-Marc Nicaud; Kenneth N Timmis; Peter N Golyshin

doi:10.1111/j.1574-6968.2011.02279.x

Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes

Julia S Sabirova
, Anke Becker
, Heinrich Lünsdorf
, Jean-Marc Nicaud
, Kenneth N Timmis
, Peter N Golyshin

Ghent University

Research output: Contribution to journal › Article › peer-review

Abstract

The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.

Original language	English
Pages (from-to)	160-8
Number of pages	9
Journal	FEMS Microbiology Letters
Volume	319
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2011.02279.x
Publication status	Published - Jun 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Alcanivoraceae/genetics
Alkanes/metabolism
Bacterial Proteins/genetics
Biodegradation, Environmental
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Molecular Sequence Data
Seawater/microbiology
Transcription, Genetic

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10.1111/j.1574-6968.2011.02279.x

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title = "Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes",

abstract = "The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.",

keywords = "Alcanivoraceae/genetics, Alkanes/metabolism, Bacterial Proteins/genetics, Biodegradation, Environmental, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Seawater/microbiology, Transcription, Genetic",

author = "Sabirova, \{Julia S\} and Anke Becker and Heinrich L{\"u}nsdorf and Jean-Marc Nicaud and Timmis, \{Kenneth N\} and Golyshin, \{Peter N\}",

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AU - Sabirova, Julia S

AU - Becker, Anke

AU - Lünsdorf, Heinrich

AU - Nicaud, Jean-Marc

AU - Timmis, Kenneth N

AU - Golyshin, Peter N

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AB - The marine oil-degrading bacterium Alcanivorax borkumensis SK2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. In this study, we used microarray technology to characterize the transcriptional responses of A. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 genes being upregulated and 111 genes being downregulated. Among the genes upregulated on alkanes are systems predicted to be involved in the terminal oxidation of alkanes, biofilm formation, signal transduction, and regulation.

KW - Alcanivoraceae/genetics

KW - Alkanes/metabolism

KW - Bacterial Proteins/genetics

KW - Biodegradation, Environmental

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Bacterial

KW - Molecular Sequence Data

KW - Seawater/microbiology

KW - Transcription, Genetic

U2 - 10.1111/j.1574-6968.2011.02279.x

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Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes

Abstract

UN SDGs

Keywords

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