Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus

A A Dement'ev; N F Riabchenko; I I Protasevich; P N Golyshin; A I Stepanov; V M Orlov; V N Pustovaev; A A Makarov; G P Moiseev; M Ia Karpeĭskiĭ

Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus

Translated title of the contribution: Extracellular alkaline ribonuclease of Bacillus thuringiensis var. subtoxicus

A A Dement'ev
, N F Riabchenko
, I I Protasevich
, P N Golyshin
, A I Stepanov
, V M Orlov
, V N Pustovaev
, A A Makarov
, G P Moiseev
, M Ia Karpeĭskiĭ

School of Environmental & Natural Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.

Translated title of the contribution	Extracellular alkaline ribonuclease of Bacillus thuringiensis var. subtoxicus
Original language	Russian
Pages (from-to)	1338-49
Number of pages	12
Journal	Molekuliarnaia biologiia
Volume	26
Issue number	6
Publication status	Published - 1 Nov 1992

Keywords

Amino Acid Sequence
Amino Acids/analysis
Bacillus thuringiensis/enzymology
Calorimetry
Catalysis
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Mass Spectrometry
Molecular Sequence Data
Ribonucleases/chemistry

Cite this

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title = "Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus",

abstract = "Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.",

keywords = "Amino Acid Sequence, Amino Acids/analysis, Bacillus thuringiensis/enzymology, Calorimetry, Catalysis, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Molecular Sequence Data, Ribonucleases/chemistry",

author = "Dement'ev, \{A A\} and Riabchenko, \{N F\} and Protasevich, \{I I\} and Golyshin, \{P N\} and Stepanov, \{A I\} and Orlov, \{V M\} and Pustovaev, \{V N\} and Makarov, \{A A\} and Moiseev, \{G P\} and Karpeĭskiĭ, \{M Ia\}",

year = "1992",

month = nov,

day = "1",

language = "Rwsieg",

volume = "26",

pages = "1338--49",

journal = "Molekuliarnaia biologiia",

issn = "0026-8984",

publisher = "Russian Academy of Sciences",

number = "6",

}

TY - JOUR

T1 - Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus

AU - Dement'ev, A A

AU - Riabchenko, N F

AU - Protasevich, I I

AU - Golyshin, P N

AU - Stepanov, A I

AU - Orlov, V M

AU - Pustovaev, V N

AU - Makarov, A A

AU - Moiseev, G P

AU - Karpeĭskiĭ, M Ia

PY - 1992/11/1

Y1 - 1992/11/1

N2 - Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.

AB - Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.

KW - Amino Acid Sequence

KW - Amino Acids/analysis

KW - Bacillus thuringiensis/enzymology

KW - Calorimetry

KW - Catalysis

KW - Chromatography, High Pressure Liquid

KW - Electrophoresis, Polyacrylamide Gel

KW - Mass Spectrometry

KW - Molecular Sequence Data

KW - Ribonucleases/chemistry

M3 - Erthygl

C2 - 1491677

SN - 0026-8984

VL - 26

SP - 1338

EP - 1349

JO - Molekuliarnaia biologiia

JF - Molekuliarnaia biologiia

IS - 6

ER -

Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus

Abstract

Keywords

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