TY - JOUR

T1 - Developmental organization of central neurons in the adult Drosophila ventral nervous system

AU - Shepherd, David

AU - Sahota, Virender

AU - Court, Robert

AU - Williams, Darren W.

AU - Truman, James

N1 - Engineering and Physical Sciences Research Council, Grant/Award Number: PhD Studentship; Higher Education Funding Council for Wales, Grant/Award Number: BEEA; Howard Hughes Medical Institute, Grant/Award Numbers: Senior Fellowship, Visiting Scientist Program; Howard Hughes Medical Institute; Bangor University; Higher Education Funding Council for Wales; Howard Hughes Medical Institute

PY - 2019/10/15

Y1 - 2019/10/15

N2 - We have used MARCM to reveal the adult morphology of the post embryonically produced neurons in the thoracic neuromeres of the Drosophila VNS. The work builds on previous studies of the origins of the adult VNS neurons to describe the clonal organization of the adult VNS. We present data for 58 of 66 postembryonic thoracic lineages, excluding the motor neuron producing lineages (15 and 24) which have been described elsewhere. MARCM labels entire lineages but where both A and B hemilineages survive (e.g., lineages 19, 12, 13, 6, 1, 3, 8, and 11), the two hemilineages can be discriminated and we have described each hemilineage separately. Hemilineage morphology is described in relation to the known functional domains of the VNS neuropil and based on the anatomy we are able to assign broad functional roles for each hemilineage. The data show that in a thoracic hemineuromere, 16 hemilineages are primarily involved in controlling leg movements and walking, 9 are involved in the control of wing movements, and 10 interface between both leg and wing control. The data provide a baseline of understanding of the functional organization of the adult Drosophila VNS. By understanding the morphological organization of these neurons, we can begin to define and test the rules by which neuronal circuits are assembled during development and understand the functional logic and evolution of neuronal networks.

AB - We have used MARCM to reveal the adult morphology of the post embryonically produced neurons in the thoracic neuromeres of the Drosophila VNS. The work builds on previous studies of the origins of the adult VNS neurons to describe the clonal organization of the adult VNS. We present data for 58 of 66 postembryonic thoracic lineages, excluding the motor neuron producing lineages (15 and 24) which have been described elsewhere. MARCM labels entire lineages but where both A and B hemilineages survive (e.g., lineages 19, 12, 13, 6, 1, 3, 8, and 11), the two hemilineages can be discriminated and we have described each hemilineage separately. Hemilineage morphology is described in relation to the known functional domains of the VNS neuropil and based on the anatomy we are able to assign broad functional roles for each hemilineage. The data show that in a thoracic hemineuromere, 16 hemilineages are primarily involved in controlling leg movements and walking, 9 are involved in the control of wing movements, and 10 interface between both leg and wing control. The data provide a baseline of understanding of the functional organization of the adult Drosophila VNS. By understanding the morphological organization of these neurons, we can begin to define and test the rules by which neuronal circuits are assembled during development and understand the functional logic and evolution of neuronal networks.

KW - MARCM

KW - anatomy

KW - development

KW - hemi

KW - lineage

KW - neuroblast

KW - neurogenesis

KW - neuropil

U2 - 10.1002/cne.24690

DO - 10.1002/cne.24690

M3 - Article

VL - 527

SP - 2573

EP - 2598

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 1096-9861

IS - 15

ER -