Mangroves are salt-tolerant tree species found in tropical and sub-tropical regions around the globe. Whilst also important for many roles, such as storm protection, poverty alleviation and nurseries for many commercially important fish species, at present there is a focus on their potential for climate mitigation through carbon sequestration. However, to know how much carbon can be stored, it is important to know the aboveground biomass (AGB) of localised areas to monitor global carbon sequestration and produce climate mitigation procedures as AGB values can be used to estimate carbon sequestration when used in conjuncture with region-specific mangrove carbon content values. Whilst Florida’s mangrove forests are under legal protection, there have been no recent studies of AGB and the most recent in 2004 was found to contain errors. This study aimed to produce estimates of the aboveground biomass of a Southwest Florida mangrove stand containing Rhizophora mangle, Avicennia germinans and Laguncularia racemosa. It was found that AGB ranged between 4.9-104.2 t/hectare which was consistent with other research based in Florida. The most important factors influencing Florida mangrove growth are considered to be temperature and impact of storm events such as hurricanes. However, it is unclear how future effects of climate change including rising sea levels, increased surface temperatures and increased intensity and frequency of storm events will alter AGB of the forests, and their carbon storage potential. Planned projects for the future will consider standing carbon stocks for use in international mitigation policy and estimating mangrove greenhouse gas emissions.