Understanding the effect of artificial light at night (ALAN) on biodiversity is a key research topic of the 21st Century. Evidence suggests that LED lighting may be particularly disruptive due to strong short-wavelength emissions. Spectral manipulation of LED lighting to reduce these emissions may mitigate some disturbance, although further research is required to assess its value in comparison with other techniques. The impact of LED lighting has been documented for many species, however, amphibians remain relatively under-studied. Amphibians may be particularly sensitive to the effects of ALAN due to specialised vision adapted for low-light environments and reduced mobility. Artificial lighting is known to affect many aspects of their biology, including reproduction, development, movement and foraging. Amphibians may be particularly vulnerable to LED lighting due to increased sensitivity to short-wavelength light. Despite this, the effect of spectral manipulation of existing light sources has not been tested for these species. This study was designed to explore the impact of LED lighting on anuran behaviour and assess the success of spectral manipulation as mitigation. It was hypothesised that: illumination would delay emergence from daytime refugia, spectral manipulation would partially mitigate this delay and that two species would differ in their response to lighting. In a laboratory setting, emergence likelihood and response speed under typical “cool white” LEDs, filtered white LEDs (removal of wavelengths <500nm) and ambient dark conditions were compared for Cuban treefrogs (Osteopilus septentrionalis) and American green treefrogs (Dryophytes cinereus). Generalised linear mixed models (GLMMs) were constructed to examine the effect of eight explanatory variables on observed variation. Illumination affected Cuban treefrog behaviour, but not green treefrogs. Cuban treefrogs showed two diverse activity patterns, either emerging quickly or remaining immobile, demonstrating adaptive behavioural flexibility of this species. Greater resilience to evaporative water loss may explain why green treefrog activity was not influenced by artificial illumination. The filtered light source was no less disruptive than white light, suggesting that further research is required to develop reliable mitigation for amphibian habitats in order to minimise the effect of urbanisation on declining amphibian populations.