Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • Lynn Carr
  • Muriel Golzio
    University of Toulouse
  • Rosa Orlacchio
    University of Limoges
  • Geraldine Alberola
    University of Toulouse
  • Jelena Kolosnjaj-Tabi
    University of Toulouse
  • Philippe Leveque
    University of Limoges
  • Delia Arnaud-Cormos
    University of Limoges
  • Marie-Pierre Rols
    University of Toulouse
Three-dimensional (3D) cellular models represent more realistically the complexity of in vivo tumors compared to 2D cultures. While 3D models were largely used in classical electroporation, the effects of nanosecond pulsed electric field (nsPEF) have been poorly investigated. In this study, we evaluated the biological effects induced by nsPEF on spheroid tumor model derived from the HCT-116 human colorectal carcinoma cell line. By varying the number of pulses (from 1 to 500) and the polarity (unipolar and bipolar), the response of nsPEF exposure (10 ns duration, 50 kV/cm) was assessed either immediately after the application of the pulses or over a period lasting up to 6 days. Membrane permeabilization and cellular death occurred following the application of at least 100 pulses. The extent of the response increased with the number of pulses, with a significant decrease of viability, 24 h post-exposure, when 250 and 500 pulses were applied. The effects were highly reduced when an equivalent number of bipolar pulses were delivered. This reduction was eliminated when a 100 ns interphase interval was introduced into the bipolar pulses. Altogether, our results show that nsPEF effects, previously observed at the single cell level, also occur in more realistic 3D tumor spheroids models. [Abstract copyright: Copyright © 2021 Elsevier B.V. All rights reserved.]

Allweddeiriau

Iaith wreiddiolSaesneg
Rhif yr erthygl107839
CyfnodolynBioelectrochemistry (Amsterdam, Netherlands)
Cyfrol141
Dyddiad ar-lein cynnar8 Mai 2021
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Hyd 2021
Gweld graff cysylltiadau