TY - JOUR

T1 - A rapid electrophoretic method for separating rabbit skeletal muscle myosin heavy chains at high resolution

AU - Kubis, Hans-Peter

AU - Gros, Gerolf

N1 - https://doi.org/10.1002/elps.1150180113

PY - 1997

Y1 - 1997

N2 - Abstract An electrophoretic method using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was developed which gives a high-resolution separation of the known myosin heavy chains of rabbit skeletal muscle with excellent reproducibility. The gel of 10 cm total length consists of (i) a first stacking gel of 3.5% total gel concentration (T) and pH 6.8, (ii) a first separating gel of 6.6%T and pH 8.8, (iii) a second stacking gel of 6.6%T and pH 6.8, and (iv) a second separating gel of 8.8%T and pH 8.8. With this composition, a minigel system allows separation of six myosin heavy-chain (MHC) isoforms at room temperature without cooling and within 8h. In agreement with previous reports, the isoforms appear in the sequence MHCemb, MHC IIa, MHC IId, MHCneo, MHC IIb, MHC I. A special advantage is the detectability not only of the adult but also of the embryonic and neonatal isoforms MHCemb and MHCneo.

AB - Abstract An electrophoretic method using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was developed which gives a high-resolution separation of the known myosin heavy chains of rabbit skeletal muscle with excellent reproducibility. The gel of 10 cm total length consists of (i) a first stacking gel of 3.5% total gel concentration (T) and pH 6.8, (ii) a first separating gel of 6.6%T and pH 8.8, (iii) a second stacking gel of 6.6%T and pH 6.8, and (iv) a second separating gel of 8.8%T and pH 8.8. With this composition, a minigel system allows separation of six myosin heavy-chain (MHC) isoforms at room temperature without cooling and within 8h. In agreement with previous reports, the isoforms appear in the sequence MHCemb, MHC IIa, MHC IId, MHCneo, MHC IIb, MHC I. A special advantage is the detectability not only of the adult but also of the embryonic and neonatal isoforms MHCemb and MHCneo.

KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

KW - Myosin heavy chains

KW - Adult isomyosin

KW - Developmental isomyosin

KW - Rabbit skeletal muscle

U2 - 10.1002/elps.1150180113

DO - 10.1002/elps.1150180113

M3 - Article

VL - 18

SP - 64

EP - 66

JO - ELECTROPHORESIS

JF - ELECTROPHORESIS

SN - 0173-0835

IS - 1

ER -