Applications of the comet assay in particle toxicology: air pollution and engineered nanomaterials exposure

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygl adolyguadolygiad gan gymheiriaid

Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • Peter Møller
    University of Copenhagen
  • Jette Gjerke Hemmingsen
    University of Copenhagen
  • Ditte Marie Jensen
    University of Copenhagen
  • Pernille Høgh Danielsen
    University of Copenhagen
  • Dorina Gabriela Karottki
    University of Copenhagen
  • Kim Jantzen
    University of Copenhagen
  • Martin Roursgaard
    University of Copenhagen
  • Yi Cao
    University of Copenhagen
  • Ali Kermanizadeh
    University of Copenhagen
  • Henrik Klingberg
    University of Copenhagen
  • Daniel Vest Christophersen
    University of Copenhagen
  • Lars-Georg Hersoug
    University of Copenhagen
  • Steffen Loft
    University of Copenhagen

Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively damaged DNA are seen after exposure to a range of engineered nanomaterials (ENMs), including carbon black, carbon nanotubes, fullerene C60, ZnO, silver and gold. Exposure to TiO2 has generated mixed data with regard to SB and oxidatively damaged DNA in cell cultures. Nanosilica does not seem to be associated with generation of FPG-sensitive sites in cell cultures, while large differences in SB generation between studies have been noted. Single-dose airway exposure to nanosized carbon black and multi-walled carbon nanotubes in animal models seems to be associated with elevated DNA damage levels in lung tissue in comparison to similar exposure to TiO2 and fullerene C60. Oral exposure has been associated with augmented DNA damage levels in cells of internal organs, although the doses have been typically very high. Intraveneous and intraperitoneal injection of ENMs have shown contradictory results dependent on the type of ENM and dose in each set of experiments. In conclusion, the exposure to both combustion-derived particles and ENMs is associated with increased levels of DNA damage in the comet assay. Particle size, composition and crystal structure of ENM are considered important determinants of toxicity, whereas their combined contributions to genotoxicity in the comet assay are yet to be thoroughly investigated.

Allweddeiriau

Iaith wreiddiolSaesneg
Tudalennau (o-i)67-83
Nifer y tudalennau17
CyfnodolynMutagenesis
Cyfrol30
Rhif y cyfnodolyn1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Ion 2015
Cyhoeddwyd yn allanolIe
Gweld graff cysylltiadau