TY - JOUR

T1 - Assessment of structural characteristics of regenerated cellulolytic enzyme lignin based on a mild DMSO/[Emim]OAc dissolution system from triploid of Populus tomentosa Carr.

AU - Chen, Tian-Ying

AU - Wang, Bing

AU - Shen, Xiao-Jun

AU - Li, Han-Yin

AU - Wu, Yu-Ying

AU - Wen, Jia-Long

AU - Liu, Qiuyun

AU - Sun, Run-Cang

N1 - We are grateful for the financial support of this research from the National Natural Science Foundation of China (31500486, 31430092, 31670587), and China Ministry of Education “111” project.

PY - 2017

Y1 - 2017

N2 - The structural characteristics of native lignin are essential for the further deconstruction of plant cell walls for value-added application of lignocellulosic biomass. An improved protocol of cellulolytic enzyme lignin named regenerated cellulolytic enzyme lignin (RCEL) was developed in the present study. The dissolution process of poplar wood in the DMSO/[Emim]OAc dissolution system was dynamically monitored by microscopes and Confocal Raman Microscopy (CRM). The yield of RCEL (43.0–85.3%) was significantly higher than that of control CEL (30.6%). The isolated lignins were elaborately characterized by associated carbohydrates, 2D-HSQC NMR, 31P-NMR, and GPC techniques. NMR results showed that RCELs had similar structural features as compared to CELs. The relative abundances of the major lignin linkages (β-O-4′, β–β′, β-5′, and β-1′) and linked molecules (p-hydroxybenzoate) were quantitatively compared. Subsequent CP/MAS 13C-NMR spectra of the regenerated substrates demonstrated that the structural changes of the cellulose in the substrates occurred during the dissolution and regeneration process, resulting in efficient enzymatic hydrolysis (63.2–88.7% vs. 49.5%), thus obtaining a high yield of extracted lignin (RCEL). In short, the understanding of native lignin in fast-growing poplar will contribute to the diversification of the biomass feedstock supply for designing effective deconstruction strategies for lignocellulosic biomass.

AB - The structural characteristics of native lignin are essential for the further deconstruction of plant cell walls for value-added application of lignocellulosic biomass. An improved protocol of cellulolytic enzyme lignin named regenerated cellulolytic enzyme lignin (RCEL) was developed in the present study. The dissolution process of poplar wood in the DMSO/[Emim]OAc dissolution system was dynamically monitored by microscopes and Confocal Raman Microscopy (CRM). The yield of RCEL (43.0–85.3%) was significantly higher than that of control CEL (30.6%). The isolated lignins were elaborately characterized by associated carbohydrates, 2D-HSQC NMR, 31P-NMR, and GPC techniques. NMR results showed that RCELs had similar structural features as compared to CELs. The relative abundances of the major lignin linkages (β-O-4′, β–β′, β-5′, and β-1′) and linked molecules (p-hydroxybenzoate) were quantitatively compared. Subsequent CP/MAS 13C-NMR spectra of the regenerated substrates demonstrated that the structural changes of the cellulose in the substrates occurred during the dissolution and regeneration process, resulting in efficient enzymatic hydrolysis (63.2–88.7% vs. 49.5%), thus obtaining a high yield of extracted lignin (RCEL). In short, the understanding of native lignin in fast-growing poplar will contribute to the diversification of the biomass feedstock supply for designing effective deconstruction strategies for lignocellulosic biomass.

U2 - 10.1039/C6RA25663E

DO - 10.1039/C6RA25663E

M3 - Article

VL - 7

SP - 3376

EP - 3387

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 6

ER -