Bacteriophages pass through candle-shaped porous ceramic filters: Application for the collection of viruses in soil water

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

Fersiynau electronig

  • Perrine Florent
    Luxembourg Institute of Science and Technology
  • Henry-Michel Cauchie
    Luxembourg Institute of Science and Technology
  • Malte Herold
    Luxembourg Institute of Science and Technology
  • Leslie Ogorzaly
    Luxembourg Institute of Science and Technology
Despite the ubiquity of viruses in soils, their diversity in soil water has not been explored, mainly due to the difficulty of collecting them. In hydrology, soil water is usually collected using porous candles. This study proposes using these porous candles as a new tool for sampling viruses in soil water to analyze their passage through the ceramic part of the candles. The recovery of the viruses was determined after filtration under laboratory conditions using three model bacteriophages (MS2, ΦX174, and Φ6) and Escherichia coli, at neutral and acidic pH. Then, a field experiment was carried out where soil water filtration and viral identification by metagenomic shotgun were performed. At neutral pH, all bacteriophages tested successfully passed through the porous candles during the filtration process, with reductions of 0.02 log, 0.16 log, and 0.55 log for MS2 ΦX174 and Φ6, respectively. At pH 4.4, the passage of MS2 was not affected while ΦX174 underwent a slight reduction in recovery, probably caused by adsorption onto the filter material. Regarding the application of the porous candles in the field, the results obtained allowed the successful recovery of viruses, exposing porous candles as a new method suitable for the collection of viruses from soil water in the context of the study of viral communities.
Iaith wreiddiolSaesneg
Rhif y cyfnodolyn5
Dyddiad ar-lein cynnar20 Medi 2022
StatwsCyhoeddwyd - 1 Hyd 2022

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