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  • Complex target SELEX-based identification of DNA aptamers_2021

    Llawysgrif awdur wedi’i dderbyn, 1.98 MB, dogfen-PDF

    Embargo yn dod i ben: 24/07/22

    Trwydded: CC BY-NC-ND Dangos trwydded

Dangosydd eitem ddigidol (DOI)

  • Anjali Anand
    Translational Health Science and Technology 9 Institute (THSTI)
  • Bandhan Chatterjee
    Translational Health Science and Technology 9 Institute (THSTI)
  • Abhijeet Dhiman
    Translational Health Science and Technology 9 Institute (THSTI)
  • Renu Goel
    Translational Health Science and Technology 9 Institute (THSTI)
  • Eshan Khan
    Indian Institute of Technology,Indore
  • Anita Malhotra
  • Vishal Santra
    Simultala Conservationists (Foundation for Wildlife), Nalikul
  • Nitin Salvi
    Premium Serums and Vaccines Pvt. Ltd,
  • Ira Bhatnagar
    Centre for Cellular and Molecular Biology
  • Amit Kumar
    Indian Institute of Technology,Indore
  • Amit Asthana
    Centre for Cellular and Molecular Biology
  • Tarun Kumar Sharma
    Translational Health Science and Technology 9 Institute (THSTI)
Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is β-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.

Allweddeiriau

Iaith wreiddiolSaesneg
Rhif yr erthygl113523
CyfnodolynBiosensors and Bioelectronics
Cyfrol193
Dyddiad ar-lein cynnar24 Gorff 2021
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsE-gyhoeddi cyn argraffu - 24 Gorff 2021
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