TY - GEN

T1 - Degradable and thermal responsive hyperbranched polymers synthesized via one-pot RAFT polymerisation for injectable scaffold applications

AU - Tai, Hongyun

AU - Tochwin, Anna

AU - EL-Betany, A.

AU - Wang, W.

N1 - 2015 4th TERMIS World Congress Boston, Massachusetts September 8–11, 2015

PY - 2015/9/11

Y1 - 2015/9/11

N2 - In tissue engineering, injectable scaffolds offer the possibility of homogeneously distributing cells and therapeutic molecules throughout thescaffolds, and can be injected directly into cavities with irregular shapes and sizes. However, the challenge lies in finding suitable materialswhich can solidify insituto form 3D microenvironments with the desired mechanical and biological properties. Crosslinkable and smartpolymers, which change in response to external stimuli such as temperature, pH and enzyme, have attracted much attention for suchapplications. The objective of this work is to develop injectable biodegradable hydrogels from hyperbranched polymers (HBP) with responsive,crosslinkable and degradable properties. These injectable hydrogels can have tailored mechanical properties and functionalities for celladhesion via decorating with peptide motifs and for controlled release of therapeutic drugs. Here, we report the synthesis of newthermoresponsive HBP via onepotreversible addition fragmentation chain transfer (RAFT) copolymerization of poly (ethylene glycol) methylether methacrylate (PEGMEMA, Mn = 475), poly (propylene glycol) methacrylate (PPGMA, Mn = 375) and disulphide diacrylate (DSDA) using2cyanoprop2yldithiobenzoate as RAFT agent. Enzymatic degradable DSDA was used as the branching agent; PEGMEMA and PPGMAwere used as macromers to balance hydrophilicity/hydrophobicity thus obtain desired low critical solution temperatures (LCST). The resultedHBP were characterised by NMR and GPC, and LCSTs were also determined. Moreover, the studies on the degradability and swellingproperties of hydrogels prepared by the resultant HBP were conducted. The experimental results demonstrated that these degradable andthermoresponsive HBP can be used as tissue engineering injectable scaffolds.

AB - In tissue engineering, injectable scaffolds offer the possibility of homogeneously distributing cells and therapeutic molecules throughout thescaffolds, and can be injected directly into cavities with irregular shapes and sizes. However, the challenge lies in finding suitable materialswhich can solidify insituto form 3D microenvironments with the desired mechanical and biological properties. Crosslinkable and smartpolymers, which change in response to external stimuli such as temperature, pH and enzyme, have attracted much attention for suchapplications. The objective of this work is to develop injectable biodegradable hydrogels from hyperbranched polymers (HBP) with responsive,crosslinkable and degradable properties. These injectable hydrogels can have tailored mechanical properties and functionalities for celladhesion via decorating with peptide motifs and for controlled release of therapeutic drugs. Here, we report the synthesis of newthermoresponsive HBP via onepotreversible addition fragmentation chain transfer (RAFT) copolymerization of poly (ethylene glycol) methylether methacrylate (PEGMEMA, Mn = 475), poly (propylene glycol) methacrylate (PPGMA, Mn = 375) and disulphide diacrylate (DSDA) using2cyanoprop2yldithiobenzoate as RAFT agent. Enzymatic degradable DSDA was used as the branching agent; PEGMEMA and PPGMAwere used as macromers to balance hydrophilicity/hydrophobicity thus obtain desired low critical solution temperatures (LCST). The resultedHBP were characterised by NMR and GPC, and LCSTs were also determined. Moreover, the studies on the degradability and swellingproperties of hydrogels prepared by the resultant HBP were conducted. The experimental results demonstrated that these degradable andthermoresponsive HBP can be used as tissue engineering injectable scaffolds.

M3 - Conference contribution

SN - 2152-4947

VL - 21

BT - Tissue Engineering

PB - Mary Ann Liebert, Inc.

ER -