TY - JOUR

T1 - Effect of fertilizer type on antibiotic resistance genes by reshaping the bacterial community and soil properties

AU - Wu, Jie

AU - Guo, Shumin

AU - Li, Kejie

AU - Li, Zhutao

AU - Xu, Pinshang

AU - Jones, Davey L

AU - Wang, Jinyang

AU - Zou, Jianwen

N1 - Copyright © 2023 Elsevier Ltd. All rights reserved.

PY - 2023/9/1

Y1 - 2023/9/1

N2 - Conventional and bio-organic fertilizers play an important role in maintaining soil health and promoting crop growth. However, the effect of organic fertilizers on the prevalence of antibiotic resistance genes (ARGs) in the vegetable cropping system has been largely overlooked. In this study, we investigated the impacts of soil properties and biotic factors on ARG profiles by analyzing ARG and bacterial communities in vegetable copping soils with a long-term history of manure and bio-organic fertilizer application. The ARG abundance in the soil was significantly increased by 116% with manure application compared to synthetic NPK fertilizer application. This finding was corroborated by our meta-analysis that the longer the duration of manure application, the greater the response of increased soil ARG abundance. However, bio-organic fertilizers containing Trichoderma spp. Significantly reduced ARG contamination by 31% compared to manure application. About half of the ARG variation was explained by changes in bacterial abundance and structure, followed by soil properties. The mitigation of ARG by Trichoderma spp. Is achieved by altering the structure of the bacterial community and weakening the close association between bacteria and ARG prevalence. Taken together, these findings shed light on the contribution of bio-organic fertilizers in mitigating ARG contamination in agricultural soils, which can help manage the ecological risk posed by ARG inputs associated with manure application.

AB - Conventional and bio-organic fertilizers play an important role in maintaining soil health and promoting crop growth. However, the effect of organic fertilizers on the prevalence of antibiotic resistance genes (ARGs) in the vegetable cropping system has been largely overlooked. In this study, we investigated the impacts of soil properties and biotic factors on ARG profiles by analyzing ARG and bacterial communities in vegetable copping soils with a long-term history of manure and bio-organic fertilizer application. The ARG abundance in the soil was significantly increased by 116% with manure application compared to synthetic NPK fertilizer application. This finding was corroborated by our meta-analysis that the longer the duration of manure application, the greater the response of increased soil ARG abundance. However, bio-organic fertilizers containing Trichoderma spp. Significantly reduced ARG contamination by 31% compared to manure application. About half of the ARG variation was explained by changes in bacterial abundance and structure, followed by soil properties. The mitigation of ARG by Trichoderma spp. Is achieved by altering the structure of the bacterial community and weakening the close association between bacteria and ARG prevalence. Taken together, these findings shed light on the contribution of bio-organic fertilizers in mitigating ARG contamination in agricultural soils, which can help manage the ecological risk posed by ARG inputs associated with manure application.

KW - Soil/chemistry

KW - Anti-Bacterial Agents/pharmacology

KW - Fertilizers/analysis

KW - Genes, Bacterial

KW - Manure/microbiology

KW - Soil Microbiology

KW - Bacteria/genetics

KW - Drug Resistance, Microbial/genetics

KW - Vegetables/genetics

U2 - 10.1016/j.chemosphere.2023.139272

DO - 10.1016/j.chemosphere.2023.139272

M3 - Article

C2 - 37343633

VL - 336

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

M1 - 139272

ER -