TY - JOUR

T1 - Effect of oral glutamine supplementation on human neutrophil lipopolysaccharide-stimulated degranulation following prolonged exercise

AU - Walsh, N P

AU - Blannin, Andrew K.

AU - Bishop, Nicolette C.

AU - Robson, Paula J.

AU - Gleeson, Michael

PY - 2000/3

Y1 - 2000/3

N2 - Recent studies have shown that neutrophils can utilize glutamine and that glutamine supplementation can improve neutrophil function in postoperative and burn patients. The present study investigated the influence of oral glutamine supplementation on stimulated neutrophil degranulation and oxidative burst activity following prolonged exercise. Subjects, 7 well-trained men, reported to the laboratory following an overnight fast and cycled for 2 hrs at 60% VO2max on two occasions a week apart. They were randomly assigned to either a glutamine or placebo treatment. For both trials, subjects consumed a sugar-free lemon drink at 15-min intervals until 90 minutes, then a lemon flavored glutamine drink (GLN) or sugar-free lemon drink (PLA) was consumed at 15-min intervals for the remaining exercise and the 2-hr recovery period. Venous blood samples were taken pre-, during, and postexercise. Glutamine supplementation had no effect on the magnitude of postexercise leukocytosis, the plasma elastase concentration following exercise (which increased in both trials), or the plasma elastase release in response to bacterial stimulation (which fell in both trials). Neutrophil function assessed by oxidative burst activity of isolated cells did not change following exercise in either trial. These findings therefore suggest that the fall in plasma glutamine concentration does not account for the decrease in neutrophil function (degranulation response) following prolonged exercise.

AB - Recent studies have shown that neutrophils can utilize glutamine and that glutamine supplementation can improve neutrophil function in postoperative and burn patients. The present study investigated the influence of oral glutamine supplementation on stimulated neutrophil degranulation and oxidative burst activity following prolonged exercise. Subjects, 7 well-trained men, reported to the laboratory following an overnight fast and cycled for 2 hrs at 60% VO2max on two occasions a week apart. They were randomly assigned to either a glutamine or placebo treatment. For both trials, subjects consumed a sugar-free lemon drink at 15-min intervals until 90 minutes, then a lemon flavored glutamine drink (GLN) or sugar-free lemon drink (PLA) was consumed at 15-min intervals for the remaining exercise and the 2-hr recovery period. Venous blood samples were taken pre-, during, and postexercise. Glutamine supplementation had no effect on the magnitude of postexercise leukocytosis, the plasma elastase concentration following exercise (which increased in both trials), or the plasma elastase release in response to bacterial stimulation (which fell in both trials). Neutrophil function assessed by oxidative burst activity of isolated cells did not change following exercise in either trial. These findings therefore suggest that the fall in plasma glutamine concentration does not account for the decrease in neutrophil function (degranulation response) following prolonged exercise.

KW - Administration, Oral

KW - Adult

KW - Analysis of Variance

KW - Blood Glucose

KW - Burns

KW - Cell Degranulation

KW - Dietary Supplements

KW - Exercise Test

KW - Fasting

KW - Follow-Up Studies

KW - Glutamine

KW - Heart Rate

KW - Humans

KW - Leukocytosis

KW - Lipopolysaccharides

KW - Male

KW - Neutrophil Activation

KW - Neutrophils

KW - Oxygen Consumption

KW - Pancreatic Elastase

KW - Physical Exertion

KW - Placebos

KW - Respiratory Burst

KW - Surgical Procedures, Operative

KW - Clinical Trial

KW - Comparative Study

KW - Journal Article

KW - Randomized Controlled Trial

KW - Research Support, Non-U.S. Gov't

U2 - 10.1123/ijsnem.10.1.39

DO - 10.1123/ijsnem.10.1.39

M3 - Article

C2 - 10722780

VL - 10

SP - 39

EP - 50

JO - International Journal of Sport Nutrition and Exercise Metabolism

JF - International Journal of Sport Nutrition and Exercise Metabolism

SN - 1526-484X

IS - 1

ER -