TY - JOUR

T1 - Exercise intensity and duration effects on in vivo immunity.

AU - Diment, B.C.

AU - Fortes, M.B.

AU - Edwards, J.P.

AU - Hanstock, H.G.

AU - Ward, M.D.

AU - Dunstall, H.M.

AU - Friedmann, P.S.

AU - Walsh, N.P.

N1 - This is a non-final version of an article published in final form in Medicine & Science in Sports & Exercise

PY - 2014/11/3

Y1 - 2014/11/3

N2 - Purpose: To examine the effects of intensity and duration of exercise stress on induction of in-vivo immunity in humans using experimental contact hypersensitivity (CHS) with the novel antigen Diphenylcyclopropenone (DPCP). Methods: Sixty-four healthy males completed either 30 minutes running at 60% V[spacing dot above]O2peak (30MI); 30 minutes running at 80% V[spacing dot above]O2peak (30HI); 120 minutes running at 60% V[spacing dot above]O2peak (120MI) or seated rest (CON). Twenty-minutes later subjects received a sensitizing dose of DPCP and four-weeks later the strength of immune reactivity was quantified by measuring the cutaneous responses to a low, dose-series challenge with DPCP on the upper inner-arm. Circulating epinephrine, norepinephrine and cortisol were measured pre, post and 1h post-exercise or CON. Next, to better understand whether the decrease in CHS response on 120MI was due to local inflammatory or T-cell mediated processes, in a cross-over design, eleven healthy males performed 120MI and CON and cutaneous responses to a dose-series of the irritant, croton oil (CO) were assessed on the upper inner-arm. Results: Immune induction by DPCP was impaired by 120MI (skin-fold-thickness -67% vs CON; P

AB - Purpose: To examine the effects of intensity and duration of exercise stress on induction of in-vivo immunity in humans using experimental contact hypersensitivity (CHS) with the novel antigen Diphenylcyclopropenone (DPCP). Methods: Sixty-four healthy males completed either 30 minutes running at 60% V[spacing dot above]O2peak (30MI); 30 minutes running at 80% V[spacing dot above]O2peak (30HI); 120 minutes running at 60% V[spacing dot above]O2peak (120MI) or seated rest (CON). Twenty-minutes later subjects received a sensitizing dose of DPCP and four-weeks later the strength of immune reactivity was quantified by measuring the cutaneous responses to a low, dose-series challenge with DPCP on the upper inner-arm. Circulating epinephrine, norepinephrine and cortisol were measured pre, post and 1h post-exercise or CON. Next, to better understand whether the decrease in CHS response on 120MI was due to local inflammatory or T-cell mediated processes, in a cross-over design, eleven healthy males performed 120MI and CON and cutaneous responses to a dose-series of the irritant, croton oil (CO) were assessed on the upper inner-arm. Results: Immune induction by DPCP was impaired by 120MI (skin-fold-thickness -67% vs CON; P

U2 - 10.1249/MSS.0000000000000562

DO - 10.1249/MSS.0000000000000562

M3 - Article

VL - 47

SP - 1390

EP - 1398

JO - Medicine and Science in Sports and Exercise

JF - Medicine and Science in Sports and Exercise

SN - 0195-9131

IS - 7

ER -