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  • 2019 bdexpression_preprint

    Llawysgrif awdur wedi’i dderbyn, 7 MB, dogfen-PDF

    Embargo yn dod i ben: 31/12/99

Dangosydd eitem ddigidol (DOI)

  • C. A. McDonald
    Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY
  • Amy Ellison
  • L. F. Toledo
    Universidade Estadual de Campinas
  • T. Y. James
    University of Michigan
  • Kelly R. Zamudio
    Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY
While much research focus is paid to hypervirulent fungal lineages during emerging infectious disease outbreaks, examining enzootic pathogen isolates can be equally fruitful in delineating infection dynamics and determining pathogenesis. The fungal pathogen of amphibians, Batrachochytrium dendrobatidis (Bd), exhibits markedly different patterns of disease in natural populations, where it has caused massive amphibian declines in some regions, yet persists enzootically in others. Here we compare in vitro gene expression profiles of a panel of Bd isolates representing both the enzootic Bd-Brazil lineage, and the more recently diverged, panzootic lineage, Bd-GPL. We document significantly different lineage-specific and intralineage gene expression patterns, with Bd-Brazil upregulating genes with aspartic-type peptidase activity, and Bd-GPL upregulating CBM18 chitin-binding genes, among others. We also find pronounced intralineage variation in membrane integrity and transmembrane transport ability within our Bd-GPL isolates. Finally, we highlight unexpectedly divergent expression profiles in sympatric panzootic isolates, underscoring microgeographic functional variation in a largely clonal lineage. This variation in gene expression likely plays an important role in the relative pathogenesis and host range of Bd-Brazil and Bd-GPL isolates. Together, our results demonstrate that functional genomics approaches can provide information relevant to studies of virulence evolution within the Bd clade.
Iaith wreiddiolSaesneg
CyfnodolynFungal Biology
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsWedi ei Dderbyn / Yn y wasg - 24 Hyd 2019
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