TY - JOUR

T1 - Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator.

AU - Colson, Isabelle

AU - Hartsuiker, Edgar

PY - 2018/2

Y1 - 2018/2

N2 - n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.

AB - n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.

U2 - 10.1101/pdb.prot091967

DO - 10.1101/pdb.prot091967

M3 - Article

VL - 2018

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1559-6095

IS - 2

ER -