Genome-wide discovery of OsHOX24-binding sites and regulation of desiccation stress response in rice
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Yn: Plant Molecular Biology, Cyfrol 105, Rhif 1-2, 01.01.2021, t. 205-214.
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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T1 - Genome-wide discovery of OsHOX24-binding sites and regulation of desiccation stress response in rice
AU - Bhattacharjee, Annapurna
AU - Srivastava, Prabhakar Lal
AU - Nath, Onkar
AU - Jain, Mukesh
PY - 2021/1/1
Y1 - 2021/1/1
N2 - OsHOX24 mediates regulation of desiccation stress response via complex regulatory network as indicated by its binding to several target genes including transcription factors in rice. HD-ZIP I subfamily of homeobox transcription factors (TFs) are involved in abiotic stress responses and plant development. Previously, we demonstrated the role of OsHOX24, a member of HD-ZIP I subfamily, in abiotic stress responses. In this study, we identified downstream targets of OsHOX24 under control and desiccation stress conditions via chromatin immunoprecipitation-sequencing (ChIP-seq) approach in wild-type and OsHOX24 over-expression transgenic in rice. OsHOX24-binding sites in each sample and differential binding sites between the samples were detected at various genomic locations, including genic and intergenic regions. Gene ontology enrichment analysis revealed that OsHOX24 direct target genes were involved in several biological processes, including plant development, ABA-mediated signalling pathway, ubiquitin-dependent protein catabolic process, ion transport, abiotic and biotic stress responses besides transcriptional and translational regulation. The enrichment of several cis-regulatory motifs representing binding sites of other TFs, such as ABFs, ERF1, MYB1, LTREs and SORLIP2, suggested the involvement of OsHOX24 in a complex regulatory network. These findings indicate that OsHOX24-mediated desiccation stress regulation involves modulation of a plethora of target genes, which participate in diverse pathways in rice.
AB - OsHOX24 mediates regulation of desiccation stress response via complex regulatory network as indicated by its binding to several target genes including transcription factors in rice. HD-ZIP I subfamily of homeobox transcription factors (TFs) are involved in abiotic stress responses and plant development. Previously, we demonstrated the role of OsHOX24, a member of HD-ZIP I subfamily, in abiotic stress responses. In this study, we identified downstream targets of OsHOX24 under control and desiccation stress conditions via chromatin immunoprecipitation-sequencing (ChIP-seq) approach in wild-type and OsHOX24 over-expression transgenic in rice. OsHOX24-binding sites in each sample and differential binding sites between the samples were detected at various genomic locations, including genic and intergenic regions. Gene ontology enrichment analysis revealed that OsHOX24 direct target genes were involved in several biological processes, including plant development, ABA-mediated signalling pathway, ubiquitin-dependent protein catabolic process, ion transport, abiotic and biotic stress responses besides transcriptional and translational regulation. The enrichment of several cis-regulatory motifs representing binding sites of other TFs, such as ABFs, ERF1, MYB1, LTREs and SORLIP2, suggested the involvement of OsHOX24 in a complex regulatory network. These findings indicate that OsHOX24-mediated desiccation stress regulation involves modulation of a plethora of target genes, which participate in diverse pathways in rice.
KW - Binding Sites
KW - Biological Phenomena
KW - Desiccation
KW - Gene Expression Regulation, Plant
KW - Genes, Plant
KW - Genome-Wide Association Study
KW - Oryza/genetics
KW - Plant Proteins/genetics
KW - Plants, Genetically Modified/genetics
KW - Promoter Regions, Genetic
KW - Seedlings
KW - Stress, Physiological/genetics
KW - Transcription Factors/genetics
KW - Transcriptome
U2 - 10.1007/s11103-020-01078-9
DO - 10.1007/s11103-020-01078-9
M3 - Article
C2 - 33025523
VL - 105
SP - 205
EP - 214
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 1-2
ER -