Wastewater effluents from mines and metal refineries are often contaminated with heavy metal ions, so they pose hazards to human and environmental health. Conventional technologies to remove heavy metal ions are well-established, but the most popular methods have drawbacks: chemical precipitation generates sludge waste, and activated carbon and ion exchange resins are made from unsustainable non-renewable resources. Using microbial biomass as the platform for heavy metal ion removal is an alternative method. Specifically, bioaccumulation is a natural biological phenomenon where microorganisms use proteins to uptake and sequester metal ions in the intracellular space to utilize in cellular processes (e.g. enzyme catalysis, signaling, stabilizing charges on biomolecules). Recombinant expression of these import-storage systems in genetically engineered microorganisms allows for enhanced uptake and sequestration of heavy metal ions. This has been studied for over two decades for bioremediative
applications, but successful translation to industrial-scale processes is virtually non-existent. Meanwhile, demands for metal
resources are increasing while discovery rates to supply primary grade ores are not. This review re-thinks how bioaccumulation
can be used and proposes that it can be developed for bioextractive applications – the removal and recovery of heavy metal ions
for downstream purification and refining, rather than disposal. This review consolidates previously tested import-storage
systems into a biochemical framework and highlights efforts to overcome obstacles that limit industrial feasibility, thereby
identifying gaps in knowledge and potential avenues of research in bioaccumulation.