TY - JOUR

T1 - Hexose/H+ symporters in lower and higher plants

AU - Caspari, Thomas

AU - Will, Andreas

AU - Opekarová, Miroslava

AU - Sauer, Norbert

AU - Tanner, Widmar

PY - 1994/11

Y1 - 1994/11

N2 - A well-studied transporter of plant cells is the hexose/H+ symporter of the unicellular alga Chlorella kessleri. Its properties, studied in vivo, are briefly summarized. In part, they are atypical and it has been suggested that this porter acts in an asymmetric way. Three genes coding for Chlorella hexose transport activity have been identified (HUP1, HUP2 and HUP3). HUP1 cDNA expressed in a mutant of Schizosaccharomyces pombe not transporting any D-glucose has been studied in detail. Several mutants with changed Km values for substrate were obtained, some by random polymerase chain reaction mutation and selection for decreased sensitivity towards the toxic sugar 2-deoxyglucose, some by site-directed mutagenesis. The amino acids affected clustered in the centre of the putative transmembrane helices V, VII and XI. Large families of hexose transporter genes are found in higher plants (Arabidopsis, Chenopodium, Ricinus). Their functional role is discussed. Finally, the progress made in studying plant transporters in a vesicle system energized by cytochrome c oxidase is summarized.

AB - A well-studied transporter of plant cells is the hexose/H+ symporter of the unicellular alga Chlorella kessleri. Its properties, studied in vivo, are briefly summarized. In part, they are atypical and it has been suggested that this porter acts in an asymmetric way. Three genes coding for Chlorella hexose transport activity have been identified (HUP1, HUP2 and HUP3). HUP1 cDNA expressed in a mutant of Schizosaccharomyces pombe not transporting any D-glucose has been studied in detail. Several mutants with changed Km values for substrate were obtained, some by random polymerase chain reaction mutation and selection for decreased sensitivity towards the toxic sugar 2-deoxyglucose, some by site-directed mutagenesis. The amino acids affected clustered in the centre of the putative transmembrane helices V, VII and XI. Large families of hexose transporter genes are found in higher plants (Arabidopsis, Chenopodium, Ricinus). Their functional role is discussed. Finally, the progress made in studying plant transporters in a vesicle system energized by cytochrome c oxidase is summarized.

KW - Amino Acid Sequence

KW - Arabidopsis Proteins

KW - Carrier Proteins

KW - Chlorella

KW - Genes, Plant

KW - Hexoses

KW - Membrane Proteins

KW - Models, Biological

KW - Models, Structural

KW - Molecular Sequence Data

KW - Monosaccharide Transport Proteins

KW - Protein Structure, Secondary

KW - Species Specificity

KW - Symporters

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Review

M3 - Article

C2 - 7823042

VL - 196

SP - 483

EP - 491

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

ER -