High-Throughput Quantitative Genetic Interaction Mapping in the Fission Yeast Schizosaccharomyces pombe.

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  • Assen Roguev
    University of California, San Francisco
  • Colm J. Ryan
    University College Dublin
  • Edgar Hartsuiker
  • Nevan J. Krogan
    University of California, San Francisco
Epistasis mapping, in which the phenotype that emerges from combining pairs of mutations is measured quantitatively, is a powerful tool for unbiased study of gene function. When performed at a large scale, this approach has been used to assign function to previously uncharacterized genes, define functional
modules and pathways, and study their cross talk. These experiments rely heavily on methods for rapid sampling of binary combinations of mutant alleles by systematic generation of a series of double mutants.
Epistasis mapping technologies now exist in various model systems. Here we provide an overview of different epistasis mapping technologies, including the pombe epistasis mapper (PEM) system designed for the collection of quantitative genetic interaction data in fission yeast Schizosaccharomyces pombe.
Comprising a series of high-throughput selection steps for generation and characterization of double mutants, the PEM system has provided insight into a wide range of biological processes as well as facilitated evolutionary analysis of genetic interactomes across different species.
Iaith wreiddiolSaesneg
Tudalennau (o-i)75-81
Nifer y tudalennau7
CyfnodolynCold Spring Harbor Protocols
Cyfrol2018
Rhif y cyfnodolyn2
Dyddiad ar-lein cynnar21 Gorff 2017
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Chwef 2018
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