High-voltage 10 ns delayed paired or bipolar pulses for in vitro bioelectric experiments
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Yn: Bioelectrochemistry, Cyfrol 137, 107648, 02.2021.
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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T1 - High-voltage 10 ns delayed paired or bipolar pulses for in vitro bioelectric experiments
AU - Orlacchio, Rosa
AU - Carr, Lynn
AU - Palego, Cristiano
AU - Arnaud-Cormos, Delia
AU - Leveque, Philippe
PY - 2021/2
Y1 - 2021/2
N2 - Recent studies proved that classical bio-effects induced by nanosecond pulsed electric field (nsPEF) can be reduced by the delivery of a negative polarity pulse generated immediately after a positive polarity pulse. This phenomenon is known as “bipolar cancellation” and it was reported for a wide range of bipolar pulses with pulse duration from 2 ns to 900 ns. On the contrary, paired pulses, i.e., two identical pulses with the same polarity, increased traditional nsPEF outcomes. Herein, we propose a novel robust and flexible generator, based on the frozen-wave concept, able to produce a broad range of pulses with the duration of 10 ns and delay between 17 and 360 ns. Numerical simulations and experimental measurements were performed to fully characterize the proposed generator. YO-PROTM-1 uptake was investigated in the U87-MG human glioblastoma cell line as a marker of membrane permeabilization in response to 10 ns, 11.5 MV/m nsPEF. Our results showed that bipolar cancellation occurred for delays of 0–30 ns and decreased as a function of the interphase interval. In addition, we observed that cellular response following the application of paired nsPEF was more than two-fold compared to the unipolar pulse response and was independent from the interphase interval.
AB - Recent studies proved that classical bio-effects induced by nanosecond pulsed electric field (nsPEF) can be reduced by the delivery of a negative polarity pulse generated immediately after a positive polarity pulse. This phenomenon is known as “bipolar cancellation” and it was reported for a wide range of bipolar pulses with pulse duration from 2 ns to 900 ns. On the contrary, paired pulses, i.e., two identical pulses with the same polarity, increased traditional nsPEF outcomes. Herein, we propose a novel robust and flexible generator, based on the frozen-wave concept, able to produce a broad range of pulses with the duration of 10 ns and delay between 17 and 360 ns. Numerical simulations and experimental measurements were performed to fully characterize the proposed generator. YO-PROTM-1 uptake was investigated in the U87-MG human glioblastoma cell line as a marker of membrane permeabilization in response to 10 ns, 11.5 MV/m nsPEF. Our results showed that bipolar cancellation occurred for delays of 0–30 ns and decreased as a function of the interphase interval. In addition, we observed that cellular response following the application of paired nsPEF was more than two-fold compared to the unipolar pulse response and was independent from the interphase interval.
KW - Bipolar cancellation
KW - Interphase pulse interval
KW - Pulsed electric field
KW - Versatile nanosecond generator
KW - YO-PRO (TM)-1 uptake
KW - nsPEF
UR - https://ars.els-cdn.com/content/image/1-s2.0-S1567539420302061-mmc1.xml
U2 - 10.1016/j.bioelechem.2020.107648
DO - 10.1016/j.bioelechem.2020.107648
M3 - Article
VL - 137
JO - Bioelectrochemistry
JF - Bioelectrochemistry
SN - 1567-5394
M1 - 107648
ER -