Identification of a Class of Protein ADP-Ribosylating Sirtuins in Microbial Pathogens
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
Fersiynau electronig
Dangosydd eitem ddigidol (DOI)
Sirtuins are an ancient family of NAD(+)-dependent deacylases connected with the regulation of fundamental cellular processes including metabolic homeostasis and genome integrity. We show the existence of a hitherto unrecognized class of sirtuins, found predominantly in microbial pathogens. In contrast to earlier described classes, these sirtuins exhibit robust protein ADP-ribosylation activity. In our model organisms, Staphylococcus aureus and Streptococcus pyogenes, the activity is dependent on prior lipoylation of the target protein and can be reversed by a sirtuin-associated macrodomain protein. Together, our data describe a sirtuin-dependent reversible protein ADP-ribosylation system and establish a crosstalk between lipoylation and mono-ADP-ribosylation. We propose that these posttranslational modifications modulate microbial virulence by regulating the response to host-derived reactive oxygen species.
Allweddeiriau
Iaith wreiddiol | Saesneg |
---|---|
Tudalennau (o-i) | 309-20 |
Nifer y tudalennau | 12 |
Cyfnodolyn | Molecular Cell |
Cyfrol | 59 |
Rhif y cyfnodolyn | 2 |
Dyddiad ar-lein cynnar | 9 Gorff 2015 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 16 Gorff 2015 |
Cyhoeddwyd yn allanol | Ie |