Fersiynau electronig

Dogfennau

Dangosydd eitem ddigidol (DOI)

  • Lolita Ammann
    Agroscope, Zurich
  • Rosemary Moorhouse-Gann
    School of Healthcare Sciences, Cardiff University
  • Colette Bertrand
    Agroscope, Zurich
  • Laia Mestre
    University of Koblenz-Landau
  • Jordan Cuff
    School of Healthcare Sciences, Cardiff University
  • Nicolas Perez Hidalgo
    Instituto de Biologia Integrativa de Sistemas
  • Amy Ellison
  • Felix Herzog
    Agroscope, Zurich
  • Martin Entling
    University of Koblenz-Landau
  • Matthias Albrecht
    Agroscope, Zurich
  • W.O.C. Symondson
    School of Healthcare Sciences, Cardiff University
Elucidating the diets of insect predators is important in basic and applied ecology, such as for improving the effectiveness of conservation biological control measures to promote natural enemies of crop pests. Here, we investigated the aphid diet of two common aphid predators in Central European agroecosystems, the native Coccinella septempunctata (Linnaeus) and the invasive Harmonia axyridis (Pallas; Coleoptera: Coccinellidae) by means of high throughput sequencing (HTS). For acquiring insights into diets of mobile flying insects at landscape scale minimizing trapping bias is important, which imposes methodological challenges for HTS. We therefore assessed the suitability of three field sampling methods (sticky traps, pan traps and hand-collection) as well as new aphid primers for identifying aphid prey consumption by coccinellids through HTS. The new aphid primers facilitate identification to species level in 75% of the European aphid genera investigated. Aphid primer specificity was high in silico and in vitro but low in environmental samples with the methods used, although this could be improved in future studies. Ladybird sampling with sticky traps was a suitable in terms of minimized sampling bias, contamination risk and trapping success, but compromised on DNA-recovery rate. The aphid diets of both field-captured ladybird species were dominated by Microlophium carnosum, the common nettle aphid. Another common prey was Sitobion avenae (cereal aphid), which got more often detected in C. septempunctata compared to H. axyridis. Around one third of the recovered aphid taxa were common crop pests. We conclude that sampling methodologies need constant revision but that our improved aphid primers offer currently one of the best solutions for broad screenings of coccinellid predation on aphids.
Iaith wreiddiolSaesneg
Rhif yr erthygle0235054
CyfnodolynPLoS ONE
Cyfrol15
Rhif y cyfnodolyn7
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Gorff 2020

Cyfanswm lawlrlwytho

Nid oes data ar gael
Gweld graff cysylltiadau