Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

StandardStandard

Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi. / Arruda, Bruna; George, Paul B L; Robin, Agnès et al.
Yn: Mycorrhiza, Cyfrol 31, Rhif 5, 01.10.2021, t. 545-558.

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

HarvardHarvard

Arruda, B, George, PBL, Robin, A, de L C Mescolotti, D, Herrera, WFB, Jones, DL & Andreote, FD 2021, 'Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi', Mycorrhiza, cyfrol. 31, rhif 5, tt. 545-558. https://doi.org/10.1007/s00572-021-01044-3

APA

Arruda, B., George, P. B. L., Robin, A., de L C Mescolotti, D., Herrera, W. F. B., Jones, D. L., & Andreote, F. D. (2021). Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi. Mycorrhiza, 31(5), 545-558. https://doi.org/10.1007/s00572-021-01044-3

CBE

Arruda B, George PBL, Robin A, de L C Mescolotti D, Herrera WFB, Jones DL, Andreote FD. 2021. Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi. Mycorrhiza. 31(5):545-558. https://doi.org/10.1007/s00572-021-01044-3

MLA

VancouverVancouver

Arruda B, George PBL, Robin A, de L C Mescolotti D, Herrera WFB, Jones DL et al. Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi. Mycorrhiza. 2021 Hyd 1;31(5):545-558. Epub 2021 Awst 7. doi: 10.1007/s00572-021-01044-3

Author

Arruda, Bruna ; George, Paul B L ; Robin, Agnès et al. / Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi. Yn: Mycorrhiza. 2021 ; Cyfrol 31, Rhif 5. tt. 545-558.

RIS

TY - JOUR

T1 - Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi

AU - Arruda, Bruna

AU - George, Paul B L

AU - Robin, Agnès

AU - de L C Mescolotti, Denise

AU - Herrera, Wilfrand F B

AU - Jones, Davey L

AU - Andreote, Fernando D

N1 - © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Arbuscular mycorrhizal fungi (AMF) are important symbionts of many plant species, facilitating the acquisition of soil nutrients by roots. We hypothesized that AMF root colonization is strongly influenced by the composition of the soil microbiome. Here, we evaluated mycorrhizal colonization of two plants, the grass Urochloa brizantha (Brachiaria) and the legume Crotalaria juncea (Crotalaria). These were cultivated in the same soil but hosting eight distinct microbiomes: natural soil (i); soil exposed to heat treatments for 1 h at 50 ºC (ii), 80 ºC (iii), or 100 ºC (iv); sterilized soil by autoclaving (AS) followed by re-inoculation of dilutions of the natural soil community at 10-1 (v), 10-3 (vi), and 10-6 (vii); and AS without re-inoculation (viii). Microbial diversity (bacteria and fungi) was assessed through 16S rDNA and ITS1 metabarcoding, respectively, and the soil acid phosphatase activity (APASE) was measured. Sequencing results showed the formation of distinct microbial communities according to the soil manipulations, which also correlated with the decline of APASE. Subsequently, seedlings of Brachiaria and Crotalaria were grown in those soils inoculated separately with three AMF (Acaulospora colombiana, Rhizophagus clarus, and Dentiscutata heterogama) which were compared to an AMF-free control treatment. Brachiaria showed higher colonization in natural soil when compared to the microbial community manipulations, regardless of the AMF species inoculated. In contrast, two mycorrhiza species were able to colonize Crotalaria under modified microbial communities at similar rates to natural soil. Furthermore, Brachiaria showed a possible inverse relationship between APASE and mycorrhization, but this trend was absent for Crotalaria. We conclude that mycorrhizal root colonization and soil acid phosphatase activity were associated with the structure of the soil microbiome, depending on the plant species evaluated.

AB - Arbuscular mycorrhizal fungi (AMF) are important symbionts of many plant species, facilitating the acquisition of soil nutrients by roots. We hypothesized that AMF root colonization is strongly influenced by the composition of the soil microbiome. Here, we evaluated mycorrhizal colonization of two plants, the grass Urochloa brizantha (Brachiaria) and the legume Crotalaria juncea (Crotalaria). These were cultivated in the same soil but hosting eight distinct microbiomes: natural soil (i); soil exposed to heat treatments for 1 h at 50 ºC (ii), 80 ºC (iii), or 100 ºC (iv); sterilized soil by autoclaving (AS) followed by re-inoculation of dilutions of the natural soil community at 10-1 (v), 10-3 (vi), and 10-6 (vii); and AS without re-inoculation (viii). Microbial diversity (bacteria and fungi) was assessed through 16S rDNA and ITS1 metabarcoding, respectively, and the soil acid phosphatase activity (APASE) was measured. Sequencing results showed the formation of distinct microbial communities according to the soil manipulations, which also correlated with the decline of APASE. Subsequently, seedlings of Brachiaria and Crotalaria were grown in those soils inoculated separately with three AMF (Acaulospora colombiana, Rhizophagus clarus, and Dentiscutata heterogama) which were compared to an AMF-free control treatment. Brachiaria showed higher colonization in natural soil when compared to the microbial community manipulations, regardless of the AMF species inoculated. In contrast, two mycorrhiza species were able to colonize Crotalaria under modified microbial communities at similar rates to natural soil. Furthermore, Brachiaria showed a possible inverse relationship between APASE and mycorrhization, but this trend was absent for Crotalaria. We conclude that mycorrhizal root colonization and soil acid phosphatase activity were associated with the structure of the soil microbiome, depending on the plant species evaluated.

KW - Fabaceae

KW - Fungi

KW - Microbiota

KW - Mycorrhizae

KW - Plant Roots

KW - Soil

KW - Soil Microbiology

U2 - 10.1007/s00572-021-01044-3

DO - 10.1007/s00572-021-01044-3

M3 - Article

C2 - 34363527

VL - 31

SP - 545

EP - 558

JO - Mycorrhiza

JF - Mycorrhiza

SN - 0940-6360

IS - 5

ER -