We report on the first demonstration of a living biological probe that is utilized to measure microscale vibration information. The microsphere-based living biological probes were precisely manipulated through optical fiber tweezers technology, enabling to transmission and focus of the emitted light on the surface of the vibrating microstructure and then combining it with laser self-mixing interference to obtain the microscale vibration information. Experiments show that the living probes can successfully detect the microscale vibration information of microstructure, and the relationship between the interference fringe number of the self-mixing signal and the peak-to-peak amplitude of the vibration of the microstructure was analyzed. The presented living probes may expand the application of optical fiber probes for detecting microscale vibration information in the biological field.