Methodological bias associated with soluble protein recovery from soil

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

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Methodological bias associated with soluble protein recovery from soil. / Greenfield, Lucy; Hill, Paul; Paterson, Eric et al.
Yn: Scientific Reports, Cyfrol 8, 11186 (2018), 25.07.2018.

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

HarvardHarvard

Greenfield, L, Hill, P, Paterson, E, Baggs, L & Jones, DL 2018, 'Methodological bias associated with soluble protein recovery from soil', Scientific Reports, cyfrol. 8, 11186 (2018). https://doi.org/10.1038/s41598-018-29559-4

APA

Greenfield, L., Hill, P., Paterson, E., Baggs, L., & Jones, D. L. (2018). Methodological bias associated with soluble protein recovery from soil. Scientific Reports, 8, Erthygl 11186 (2018). https://doi.org/10.1038/s41598-018-29559-4

CBE

Greenfield L, Hill P, Paterson E, Baggs L, Jones DL. 2018. Methodological bias associated with soluble protein recovery from soil. Scientific Reports. 8:Article 11186 (2018). https://doi.org/10.1038/s41598-018-29559-4

MLA

VancouverVancouver

Greenfield L, Hill P, Paterson E, Baggs L, Jones DL. Methodological bias associated with soluble protein recovery from soil. Scientific Reports. 2018 Gor 25;8:11186 (2018). doi: 10.1038/s41598-018-29559-4

Author

Greenfield, Lucy ; Hill, Paul ; Paterson, Eric et al. / Methodological bias associated with soluble protein recovery from soil. Yn: Scientific Reports. 2018 ; Cyfrol 8.

RIS

TY - JOUR

T1 - Methodological bias associated with soluble protein recovery from soil

AU - Greenfield, Lucy

AU - Hill, Paul

AU - Paterson, Eric

AU - Baggs, Liz

AU - Jones, Davey L.

PY - 2018/7/25

Y1 - 2018/7/25

N2 - Proteins play a crucial role in many soil processes, however, standardised methods to extract soluble protein from soil are lacking. The aim of this study was to compare the ability of different extractants to quantify the recovery of soluble proteins from three soil types (Cambisol, Ferralsol and Histosol) with contrasting clay and organic matter contents. Known amounts of plant-derived 14C-labelled soluble proteins were incubated with soil and then extracted with solutions of contrasting pH, concentration and polarity. Protein recovery proved highly solvent and soil dependent (Histosol > Cambisol > Ferralsol) and no single extractant was capable of complete protein recovery. In comparison to deionised water (10–60% of the total protein recovered), maximal recovery was observed with NaOH (0.1 M; 61–80%) and Na-pyrophosphate (0.05 M, pH 7.0; 45–75% recovery). We conclude that the dependence of protein recovery on both extractant and soil type prevents direct comparison of studies using different recovery methods, particularly if no extraction controls are used. We present recommendations for a standard protein extraction protocol.

AB - Proteins play a crucial role in many soil processes, however, standardised methods to extract soluble protein from soil are lacking. The aim of this study was to compare the ability of different extractants to quantify the recovery of soluble proteins from three soil types (Cambisol, Ferralsol and Histosol) with contrasting clay and organic matter contents. Known amounts of plant-derived 14C-labelled soluble proteins were incubated with soil and then extracted with solutions of contrasting pH, concentration and polarity. Protein recovery proved highly solvent and soil dependent (Histosol > Cambisol > Ferralsol) and no single extractant was capable of complete protein recovery. In comparison to deionised water (10–60% of the total protein recovered), maximal recovery was observed with NaOH (0.1 M; 61–80%) and Na-pyrophosphate (0.05 M, pH 7.0; 45–75% recovery). We conclude that the dependence of protein recovery on both extractant and soil type prevents direct comparison of studies using different recovery methods, particularly if no extraction controls are used. We present recommendations for a standard protein extraction protocol.

U2 - 10.1038/s41598-018-29559-4

DO - 10.1038/s41598-018-29559-4

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 11186 (2018)

ER -