StandardStandard

Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. / Dell'Anno, Filippo; van Zyl, Leonardo Joaquim; Trindade, Marla et al.
Yn: Environmental Pollution, Cyfrol 317, 120772, 15.01.2023.

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

HarvardHarvard

Dell'Anno, F, van Zyl, LJ, Trindade, M, Buschi, E, Cannavacciulo, A, Pepi, M, Sansone, C, Brunet, C, Ianora, A, de Pascale, D, Golyshin, P, Dell'Anno, A & Rastelli, E 2023, 'Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation', Environmental Pollution, cyfrol. 317, 120772. https://doi.org/10.1016/j.envpol.2022.120772

APA

Dell'Anno, F., van Zyl, L. J., Trindade, M., Buschi, E., Cannavacciulo, A., Pepi, M., Sansone, C., Brunet, C., Ianora, A., de Pascale, D., Golyshin, P., Dell'Anno, A., & Rastelli, E. (2023). Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. Environmental Pollution, 317, Erthygl 120772. https://doi.org/10.1016/j.envpol.2022.120772

CBE

Dell'Anno F, van Zyl LJ, Trindade M, Buschi E, Cannavacciulo A, Pepi M, Sansone C, Brunet C, Ianora A, de Pascale D, et al. 2023. Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. Environmental Pollution. 317:Article 120772. https://doi.org/10.1016/j.envpol.2022.120772

MLA

VancouverVancouver

Dell'Anno F, van Zyl LJ, Trindade M, Buschi E, Cannavacciulo A, Pepi M et al. Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. Environmental Pollution. 2023 Ion 15;317: 120772. Epub 2022 Tach 28. doi: 10.1016/j.envpol.2022.120772

Author

Dell'Anno, Filippo ; van Zyl, Leonardo Joaquim ; Trindade, Marla et al. / Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. Yn: Environmental Pollution. 2023 ; Cyfrol 317.

RIS

TY - JOUR

T1 - Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation

AU - Dell'Anno, Filippo

AU - van Zyl, Leonardo Joaquim

AU - Trindade, Marla

AU - Buschi, Emanuela

AU - Cannavacciulo, Antonio

AU - Pepi, Milva

AU - Sansone, Clementia

AU - Brunet, Christophe

AU - Ianora, Adrianna

AU - de Pascale, Donatella

AU - Golyshin, Peter

AU - Dell'Anno, Antonio

AU - Rastelli, Eugene

PY - 2023/1/15

Y1 - 2023/1/15

N2 - Petroleum hydrocarbons and heavy metals are some of the most widespread contaminants affecting marine ecosystems, urgently needing effective and sustainable remediation solutions. Microbial-based bioremediation is gaining increasing interest as an effective, economically and environmentally sustainable strategy. Here, we hypothesized that the heavily polluted coastal area facing the Sarno River mouth, which discharges >3 tons of polycyclic aromatic hydrocarbons (PAHs) and ∼15 tons of heavy metals (HMs) into the sea annually, hosts unique microbiomes including marine bacteria useful for PAHs and HMs bioremediation. We thus enriched the microbiome of marine sediments, contextually selecting for HM-resistant bacteria. The enriched mixed bacterial culture was subjected to whole-DNA sequencing, metagenome-assembled-genomes (MAGs) annotation, and further sub-culturing to obtain the major bacterial species as pure strains. We obtained two novel isolates corresponding to the two most abundant MAGs (Alcanivorax xenomutans strain-SRM1 and Halomonas alkaliantarctica strain-SRM2), and tested their ability to degrade PAHs and remove HMs. Both strains exhibited high PAHs degradation (60–100%) and HMs removal (21–100%) yield, and we described in detail >60 genes in their MAGs to unveil the possible genetic basis for such abilities. Most promising yields (∼100%) were obtained towards naphthalene, pyrene and lead. We propose these novel bacterial strains and related genetic repertoire to be further exploited for effective bioremediation of marine environments contaminated with both PAHs and HMs.

AB - Petroleum hydrocarbons and heavy metals are some of the most widespread contaminants affecting marine ecosystems, urgently needing effective and sustainable remediation solutions. Microbial-based bioremediation is gaining increasing interest as an effective, economically and environmentally sustainable strategy. Here, we hypothesized that the heavily polluted coastal area facing the Sarno River mouth, which discharges >3 tons of polycyclic aromatic hydrocarbons (PAHs) and ∼15 tons of heavy metals (HMs) into the sea annually, hosts unique microbiomes including marine bacteria useful for PAHs and HMs bioremediation. We thus enriched the microbiome of marine sediments, contextually selecting for HM-resistant bacteria. The enriched mixed bacterial culture was subjected to whole-DNA sequencing, metagenome-assembled-genomes (MAGs) annotation, and further sub-culturing to obtain the major bacterial species as pure strains. We obtained two novel isolates corresponding to the two most abundant MAGs (Alcanivorax xenomutans strain-SRM1 and Halomonas alkaliantarctica strain-SRM2), and tested their ability to degrade PAHs and remove HMs. Both strains exhibited high PAHs degradation (60–100%) and HMs removal (21–100%) yield, and we described in detail >60 genes in their MAGs to unveil the possible genetic basis for such abilities. Most promising yields (∼100%) were obtained towards naphthalene, pyrene and lead. We propose these novel bacterial strains and related genetic repertoire to be further exploited for effective bioremediation of marine environments contaminated with both PAHs and HMs.

U2 - 10.1016/j.envpol.2022.120772

DO - 10.1016/j.envpol.2022.120772

M3 - Article

VL - 317

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

M1 - 120772

ER -