Mucus: aiding elasmobranch conservation through non-invasive genetic sampling
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Yn: Endangered Species Research, Cyfrol 21, Rhif 3, 06.09.2013, t. 215-222.
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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T1 - Mucus: aiding elasmobranch conservation through non-invasive genetic sampling
AU - Lieber, Lilian
AU - Berrow, Simon
AU - Johnston, Emmett
AU - Hall, Graham
AU - Hall, Jackie
AU - Gubili, Chrysoula
AU - Sims, David W.
AU - Jones, Catherine S.
AU - Noble, Leslie R.
PY - 2013/9/6
Y1 - 2013/9/6
N2 - : Large-scale genetic sampling by non-invasive methods is of vital importance for the conservation of vulnerable or elusive species. In the marine environment, non-invasive genetic sampling can provide a powerful alternative to conventional biopsies. We designed and imple- mented mucus swabbing for a free-ranging elasmobranch, thereby demonstrating the utility ofthis method in the field. We report the first attempt at mucus collection from 30 plankton-feeding basking sharks Cetorhinus maximus from 3 spatially distinct ‘hotspots’ in Irish waters.C. maximus DNA was successfully extracted and verified using DNA barcoding of the mitochondrial DNA cytochrome c oxidase 1 gene (99% sequence similarity) and basking shark species-specific multi-plex PCRs derived from the nuclear ribosomal internal transcribed spacer 2 locus. Mitochondrial control region sequencing (1086 bp) showed that Irish samples were dominated by 2 haplotypes previously found to be globally distributed. Additionally, 1 novel haplotype was defined from western County Kerry. On-going genetic tagging will eventually provide more accurate estimates of global basking shark population structuring, abundance and behavioural ecology.
AB - : Large-scale genetic sampling by non-invasive methods is of vital importance for the conservation of vulnerable or elusive species. In the marine environment, non-invasive genetic sampling can provide a powerful alternative to conventional biopsies. We designed and imple- mented mucus swabbing for a free-ranging elasmobranch, thereby demonstrating the utility ofthis method in the field. We report the first attempt at mucus collection from 30 plankton-feeding basking sharks Cetorhinus maximus from 3 spatially distinct ‘hotspots’ in Irish waters.C. maximus DNA was successfully extracted and verified using DNA barcoding of the mitochondrial DNA cytochrome c oxidase 1 gene (99% sequence similarity) and basking shark species-specific multi-plex PCRs derived from the nuclear ribosomal internal transcribed spacer 2 locus. Mitochondrial control region sequencing (1086 bp) showed that Irish samples were dominated by 2 haplotypes previously found to be globally distributed. Additionally, 1 novel haplotype was defined from western County Kerry. On-going genetic tagging will eventually provide more accurate estimates of global basking shark population structuring, abundance and behavioural ecology.
U2 - 10.3354/esr00524
DO - 10.3354/esr00524
M3 - Article
VL - 21
SP - 215
EP - 222
JO - Endangered Species Research
JF - Endangered Species Research
SN - 1863-5407
IS - 3
ER -