Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi

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Dangosydd eitem ddigidol (DOI)

  • Sam Keyes
    University of Southampton
  • Arjen van Veelen
    University of Southampton
  • Dan McKay Fletcher
    University of Southampton
  • Callum Scotson
    University of Southampton
  • Nico Koebernick
    University of Southampton
  • Chiara Petroselli
    University of Southampton
  • Katherine Williams
    University of Southampton
  • Siul Ruiz
    University of Southampton
  • Laura Cooper
    University of Southampton
  • Robbie Mayon
    University of Southampton
  • Simon Duncan
    University of Southampton
  • Marc Dumont
    University of Southampton
  • Iver Jakobsen
    University of Copenhagen
  • Giles Oldroyd
    University of Cambridge
  • Andrzej Tkacz
    University of Oxford
  • Philip Poole
    University of Oxford
  • Fred Mosselmans
    Diamond Light Source, Harwell Science and Innovation Campus
  • Camelia Borca
    Swiss Light Source
  • Thomas Huthwelker
    Swiss Light Source
  • Davey L. Jones
  • Tiina Roose
    University of Southampton

Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated.
We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling.
We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P.
We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.

Allweddeiriau

Iaith wreiddiolSaesneg
Tudalennau (o-i)688-703
Nifer y tudalennau16
CyfnodolynNew Phytologist
Cyfrol234
Rhif y cyfnodolyn2
Dyddiad ar-lein cynnar19 Ion 2022
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Ebr 2022

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