The use of 3D imaging in the medical field has proven to be a benefit to doctors when diagnosing
patients. As for different medical applications, 3D visualization systems have advantages in terms of a better spatial
understanding of anatomical structures, better performance of tasks that require high level of dexterity, increased
learning performance, and improved communications with patients or between doctors. Methods: In this technical
report, we show how to employ a multi-view autostereoscopic system to provide 3D images without any special
glasses or equipment, describing a new way to obtain 3D visualization using sets of 2D images instead of real
volumetric data such as magnetic resonance imaging (MRI) or computed tomography (CT). We also propose an
application of the images in neuroanatomy. Results: We obtained three-dimensional images of anatomical parts
for visualization without glasses with resolution of 336 × 210 pixels’. Conclusion: The proposed method was able
to generate three-dimensional high-resolution images and has great potential to be used in various areas such as
anatomy and physiological studies.