TY - CHAP

T1 - Preparation and Utilization of a 3D Human Liver Microtissue Model for Nanotoxicological Assessment

AU - Kermanizadeh, Ali

PY - 2019

Y1 - 2019

N2 - The liver is the principal detoxification center of the body, removing xenobiotics and waste products which could potentially include some nanomaterials (NM). With the ever increasing public and occupational exposure associated with accumulative production of nanomaterials, there is an urgent need to consider the possibility of harmful health consequences of engineered NM exposure. It is understood that following exposure via inhalation, ingestion, or direct intravenous injection a fraction of NMs reach the liver. Traditional in vitro or ex vivo hepatic nanotoxicology models are often limiting and/or troublesome (i.e., reduced metabolism enzymes, lacking important cell populations, unstable with very high variability, etc.). This chapter highlights a methodology for the preparation of a physiologically relevant 3D human liver microtissue model which addresses most of the negative issues associated with the models used in traditional in vitro hepatic toxicological investigations. The spheroids are a very promising model for the assessment of the toxicological effects associated with engineered NM exposure.

AB - The liver is the principal detoxification center of the body, removing xenobiotics and waste products which could potentially include some nanomaterials (NM). With the ever increasing public and occupational exposure associated with accumulative production of nanomaterials, there is an urgent need to consider the possibility of harmful health consequences of engineered NM exposure. It is understood that following exposure via inhalation, ingestion, or direct intravenous injection a fraction of NMs reach the liver. Traditional in vitro or ex vivo hepatic nanotoxicology models are often limiting and/or troublesome (i.e., reduced metabolism enzymes, lacking important cell populations, unstable with very high variability, etc.). This chapter highlights a methodology for the preparation of a physiologically relevant 3D human liver microtissue model which addresses most of the negative issues associated with the models used in traditional in vitro hepatic toxicological investigations. The spheroids are a very promising model for the assessment of the toxicological effects associated with engineered NM exposure.

KW - Cell Culture Techniques/instrumentation

KW - Cell Line

KW - Hepatocytes

KW - Humans

KW - Liver/metabolism

KW - Nanostructures/toxicity

KW - Spheroids, Cellular

KW - Toxicity Tests/methods

U2 - 10.1007/978-1-4939-8916-4_3

DO - 10.1007/978-1-4939-8916-4_3

M3 - Chapter

C2 - 30547454

SN - 9781493989157

VL - 1894

T3 - Methods in Molecular Biology

SP - 47

EP - 55

BT - Nanotoxicity

A2 - Zhang, Qunwei

PB - Humana Press

ER -