TY - JOUR

T1 - Push–pull configuration of high-power MOSFETs for generation of nanosecond pulses for electropermeabilization of cells

AU - Davies, Ilan

AU - Merla, C.

AU - Tanori, M.

AU - Zambotti, A.

AU - Mancuso, M.

AU - Bishop, J.

AU - White, M.

AU - Palego, Cristiano

AU - Hancock, Chris

PY - 2019/9/30

Y1 - 2019/9/30

N2 - A power MOSFET-based push–pull configuration nanosecond-pulse generator has been designed, constructed, and characterized to permeabilize cells for biological and medical applications. The generator can deliver pulses with durations ranging from 80 ns up to 1 μs and pulse amplitudes up to 1.4 kV. The unit has been tested for in vitro experiments on a medulloblastoma cell line. Following the exposure of cells to 100, 200, and 300 ns electric field pulses, permeabilization tests were carried out, and viability tests were conducted to ver- ify the performance of the generator. The maximum temperature rise of the biological load was also calculated based on Joule heating energy conservation and experimental validation. Our results indicate that the developed device has good capabilities to achieve well-controlled electro-manipulation in vitro.

AB - A power MOSFET-based push–pull configuration nanosecond-pulse generator has been designed, constructed, and characterized to permeabilize cells for biological and medical applications. The generator can deliver pulses with durations ranging from 80 ns up to 1 μs and pulse amplitudes up to 1.4 kV. The unit has been tested for in vitro experiments on a medulloblastoma cell line. Following the exposure of cells to 100, 200, and 300 ns electric field pulses, permeabilization tests were carried out, and viability tests were conducted to ver- ify the performance of the generator. The maximum temperature rise of the biological load was also calculated based on Joule heating energy conservation and experimental validation. Our results indicate that the developed device has good capabilities to achieve well-controlled electro-manipulation in vitro.

U2 - 10.1017/S1759078719000576

DO - 10.1017/S1759078719000576

M3 - Article

VL - 11

SP - 645

EP - 657

JO - International Journal of Microwave and Wireless Technologies

JF - International Journal of Microwave and Wireless Technologies

SN - 1759-0795

IS - 7

ER -