Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus

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Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus. / Zhao, Bao-Cheng; Xu, Ji-Dong ; Chen, Bo-Yang et al.
Yn: Planta, Cyfrol 247, Rhif 5, 05.2018, t. 1077-1087.

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HarvardHarvard

Zhao, B-C, Xu, J-D, Chen, B-Y, Cao, X-F, Yuan, T-Q, Wang, S-F, Charlton, A & Sun, R-C 2018, 'Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus', Planta, cyfrol. 247, rhif 5, tt. 1077-1087. https://doi.org/10.1007/s00425-018-2842-9

APA

Zhao, B.-C., Xu, J.-D., Chen, B.-Y., Cao, X.-F., Yuan, T.-Q., Wang, S.-F., Charlton, A., & Sun, R.-C. (2018). Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus. Planta, 247(5), 1077-1087. https://doi.org/10.1007/s00425-018-2842-9

CBE

MLA

VancouverVancouver

Zhao BC, Xu JD, Chen BY, Cao XF, Yuan TQ, Wang SF et al. Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus. Planta. 2018 Mai;247(5):1077-1087. Epub 2018 Ion 19. doi: 10.1007/s00425-018-2842-9

Author

Zhao, Bao-Cheng ; Xu, Ji-Dong ; Chen, Bo-Yang et al. / Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus. Yn: Planta. 2018 ; Cyfrol 247, Rhif 5. tt. 1077-1087.

RIS

TY - JOUR

T1 - Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus

AU - Zhao, Bao-Cheng

AU - Xu, Ji-Dong

AU - Chen, Bo-Yang

AU - Cao, Xue-Fei

AU - Yuan, Tong-Qi

AU - Wang, Shuang-Fei

AU - Charlton, Adam

AU - Sun, Run-Cang

PY - 2018/5

Y1 - 2018/5

N2 - Six types of lignin–carbohydrate complex (LCC) fractions were isolated from Eucalyptus. The acidic dioxane treatment applied significantly improved the yield of LCCs. The extraction conditions had a limited impact on the LCC structures and linkages.Characterization of the lignin–carbohydrate complex (LCC) structures and linkages promises to offer insight on plant cell wall chemistry. In this case, Eucalyptus LCCs were extracted by aqueous dioxane, and then precipitated sequentially by 70% ethanol, 100% ethanol, and acidic water (pH = 2). The composition and structure of the six LCC fractions obtained by selective precipitation were investigated by sugar analysis, molecular weight determination, and 2D HSQC NMR. It was found that the acidic (0.05-M HCl) dioxane treatment significantly improved the yield of LCCs (66.4% based on Klason lignin), which was higher than the neutral aqueous dioxane extraction, and the extraction condition showed limited impact on the LCC structures and linkages. In the fractionation process, the low-molecular-weight LCCs containing a high content of carbohydrates (60.3–63.2%) were first precipitated by 70% ethanol from the extractable solution. The phenyl glycoside (PhGlc) bonds (13.0–17.0 per 100Ar) and highly acetylated xylans were observed in the fractions recovered by the precipitation with 100% ethanol. On the other hand, such xylan-rich LCCs exhibited the highest frequency of β-O-4 linkages. The benzyl ether (BE) bonds were only detected in the fractions obtained by acidic water precipitation.

AB - Six types of lignin–carbohydrate complex (LCC) fractions were isolated from Eucalyptus. The acidic dioxane treatment applied significantly improved the yield of LCCs. The extraction conditions had a limited impact on the LCC structures and linkages.Characterization of the lignin–carbohydrate complex (LCC) structures and linkages promises to offer insight on plant cell wall chemistry. In this case, Eucalyptus LCCs were extracted by aqueous dioxane, and then precipitated sequentially by 70% ethanol, 100% ethanol, and acidic water (pH = 2). The composition and structure of the six LCC fractions obtained by selective precipitation were investigated by sugar analysis, molecular weight determination, and 2D HSQC NMR. It was found that the acidic (0.05-M HCl) dioxane treatment significantly improved the yield of LCCs (66.4% based on Klason lignin), which was higher than the neutral aqueous dioxane extraction, and the extraction condition showed limited impact on the LCC structures and linkages. In the fractionation process, the low-molecular-weight LCCs containing a high content of carbohydrates (60.3–63.2%) were first precipitated by 70% ethanol from the extractable solution. The phenyl glycoside (PhGlc) bonds (13.0–17.0 per 100Ar) and highly acetylated xylans were observed in the fractions recovered by the precipitation with 100% ethanol. On the other hand, such xylan-rich LCCs exhibited the highest frequency of β-O-4 linkages. The benzyl ether (BE) bonds were only detected in the fractions obtained by acidic water precipitation.

U2 - 10.1007/s00425-018-2842-9

DO - 10.1007/s00425-018-2842-9

M3 - Article

VL - 247

SP - 1077

EP - 1087

JO - Planta

JF - Planta

SN - 1432-2048

IS - 5

ER -