TY - JOUR

T1 - Structural Variation of Lignin and Lignin−Carbohydrate Complex in Eucalyptus grandis × E. urophylla during Its Growth Process

AU - Zhao, Bao-Cheng

AU - Chen, Bo-Yang

AU - Yang, Sheng

AU - Yuan, Tong-Qi

AU - Charlton, Adam

AU - Sun, Run-Cang

PY - 2017

Y1 - 2017

N2 - Milled wood lignin (MWL), lignin−carbohydrate complex-rich fraction (LCC-AcOH), celluloytic enzyme lignin (CEL), and enzymatic hydrolysis residue (EHR) were sequentially isolated from Eucalyptus grandis × E. urophyllaunder mild conditions, and the variations of lignin−carbohydrate complex (LCC) linkages and lignin structures during the eucalyptus growth were investigated. The 2D HSQC NMR analysis showed that β-O-4′ and β−β′ were the mainlinkages in lignin, while other substructures were present in much lower amounts. The amounts of β-O-4′ in the MWL and LCC-AcOH fractions showed an increased tendency and those in the CEL and EHR fractions had no obvious variation with the eucalyptus growth. The S/G ratios of the MWL, LCC-AcOH, and CEL fractions increased first and then decreased, whereas those of the EHR fractions decreased with the tree age. The amount of phenyl glycoside (PhGlc) in the LCC-AcOH fractions varied consistent with the S/G ratio. The variation of the amount of benzyl ether (BE) in the MWL fractions was parallel to theS/G ratio, while that in the CEL fractions was contrary to it. These findings will provide some evidence for the structural variation of lignin and LCC in Eucalypt during its growth process.

AB - Milled wood lignin (MWL), lignin−carbohydrate complex-rich fraction (LCC-AcOH), celluloytic enzyme lignin (CEL), and enzymatic hydrolysis residue (EHR) were sequentially isolated from Eucalyptus grandis × E. urophyllaunder mild conditions, and the variations of lignin−carbohydrate complex (LCC) linkages and lignin structures during the eucalyptus growth were investigated. The 2D HSQC NMR analysis showed that β-O-4′ and β−β′ were the mainlinkages in lignin, while other substructures were present in much lower amounts. The amounts of β-O-4′ in the MWL and LCC-AcOH fractions showed an increased tendency and those in the CEL and EHR fractions had no obvious variation with the eucalyptus growth. The S/G ratios of the MWL, LCC-AcOH, and CEL fractions increased first and then decreased, whereas those of the EHR fractions decreased with the tree age. The amount of phenyl glycoside (PhGlc) in the LCC-AcOH fractions varied consistent with the S/G ratio. The variation of the amount of benzyl ether (BE) in the MWL fractions was parallel to theS/G ratio, while that in the CEL fractions was contrary to it. These findings will provide some evidence for the structural variation of lignin and LCC in Eucalypt during its growth process.

U2 - 10.1021/acssuschemeng.6b02396

DO - 10.1021/acssuschemeng.6b02396

M3 - Article

VL - 5

SP - 1113

EP - 1122

JO - ACS Sustainable Chemistry & Engineering

JF - ACS Sustainable Chemistry & Engineering

SN - 2168-0485

IS - 1

ER -