Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

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Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens. / Straube, Nicolas; Lyra, Mariana; Paijmans, Johanna et al.
Yn: Molecular Ecology Resources, 01.10.2021.

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

HarvardHarvard

Straube, N, Lyra, M, Paijmans, J, Preick, M, Basler, N, Penner, J, Rodel, M-O, Westbury, MV, Haddad, CFB, Barlow, A & Hofreiter, M 2021, 'Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens', Molecular Ecology Resources. https://doi.org/10.1111/1755-0998.13433

APA

Straube, N., Lyra, M., Paijmans, J., Preick, M., Basler, N., Penner, J., Rodel, M.-O., Westbury, M. V., Haddad, C. F. B., Barlow, A., & Hofreiter, M. (2021). Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens. Molecular Ecology Resources. https://doi.org/10.1111/1755-0998.13433

CBE

Straube N, Lyra M, Paijmans J, Preick M, Basler N, Penner J, Rodel M-O, Westbury MV, Haddad CFB, Barlow A, et al. 2021. Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens. Molecular Ecology Resources. https://doi.org/10.1111/1755-0998.13433

MLA

VancouverVancouver

Straube N, Lyra M, Paijmans J, Preick M, Basler N, Penner J et al. Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens. Molecular Ecology Resources. 2021 Hyd 1. Epub 2021 Mai 26. doi: 10.1111/1755-0998.13433

Author

Straube, Nicolas ; Lyra, Mariana ; Paijmans, Johanna et al. / Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens. Yn: Molecular Ecology Resources. 2021.

RIS

TY - JOUR

T1 - Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens

AU - Straube, Nicolas

AU - Lyra, Mariana

AU - Paijmans, Johanna

AU - Preick, Michaela

AU - Basler, Nikolas

AU - Penner, Johannes

AU - Rodel, Mark-Oliver

AU - Westbury, Michael V.

AU - Haddad, Celio F.B.

AU - Barlow, Axel

AU - Hofreiter, Michael

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology. This type of preservation damages DNA and reduces the chance of successful retrieval of genetic data. We applied ancient DNA extraction and single stranded library construction protocols to a variety of vertebrate samples obtained from wet collections and of different ages. Our results show that almost all samples tested yielded endogenous DNA. Archival DNA extraction was successful across different tissue types as well as using small amounts of tissue. Conversion of archival DNA fragments into single-stranded libraries resulted in usable data even for samples with initially undetectable DNA amounts. Subsequent target capture approaches for mitochondrial DNA using homemade baits on a subset of 30 samples resulted in almost complete mitochondrial genome sequences in several instances. Thus, application of ancient DNA methodology makes wet collection specimens, including type material as well as rare, old or extinct species, accessible for genetic and genomic analyses. Our results, accompanied by detailed step-by-step protocols, are a large step forward to open the DNA archive of museum wet collections for scientific studies

AB - Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology. This type of preservation damages DNA and reduces the chance of successful retrieval of genetic data. We applied ancient DNA extraction and single stranded library construction protocols to a variety of vertebrate samples obtained from wet collections and of different ages. Our results show that almost all samples tested yielded endogenous DNA. Archival DNA extraction was successful across different tissue types as well as using small amounts of tissue. Conversion of archival DNA fragments into single-stranded libraries resulted in usable data even for samples with initially undetectable DNA amounts. Subsequent target capture approaches for mitochondrial DNA using homemade baits on a subset of 30 samples resulted in almost complete mitochondrial genome sequences in several instances. Thus, application of ancient DNA methodology makes wet collection specimens, including type material as well as rare, old or extinct species, accessible for genetic and genomic analyses. Our results, accompanied by detailed step-by-step protocols, are a large step forward to open the DNA archive of museum wet collections for scientific studies

U2 - 10.1111/1755-0998.13433

DO - 10.1111/1755-0998.13433

M3 - Article

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

ER -