TY - JOUR

T1 - Targeted resequencing of coding DNA sequences for SNP discovery in nonmodel species

AU - Förster, Daniel W.

AU - Bull, James K.

AU - Lenz, Dorina

AU - Autenrieth, Marijke

AU - Paijmans, Johanna L. A.

AU - Kraus, Robert H. S.

AU - Nowak, Carsten

AU - Bayerl, Helmut

AU - Kuehn, Ralph

AU - Saveljev, Alexander P.

AU - Sindičić, Magda

AU - Hofreiter, Michael

AU - Schmidt, Krzysztof

AU - Fickel, Jörns

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Abstract Targeted capture coupled with high-throughput sequencing can be used to gain information about nuclear sequence variation at hundreds to thousands of loci. Divergent reference capture makes use of molecular data of one species to enrich target loci in other (related) species. This is particularly valuable for nonmodel organisms, for which often no a priori knowledge exists regarding these loci. Here, we have used targeted capture to obtain data for 809 nuclear coding DNA sequences (CDS) in a nonmodel organism, the Eurasian lynx Lynx lynx, using baits designed with the help of the published genome of a related model organism (the domestic cat Felis catus). Using this approach, we were able to survey intraspecific variation at hundreds of nuclear loci in L. lynx across the species? European range. A large set of biallelic candidate SNPs was then evaluated using a high-throughput SNP genotyping platform (Fluidigm), which we then reduced to a final 96 SNP-panel based on assay performance and reliability; validation was carried out with 100 additional Eurasian lynx samples not included in the SNP discovery phase. The 96 SNP-panel developed from CDS performed very successfully in the identification of individuals and in population genetic structure inference (including the assignment of individuals to their source population). In keeping with recent studies, our results show that genic SNPs can be valuable for genetic monitoring of wildlife species.

AB - Abstract Targeted capture coupled with high-throughput sequencing can be used to gain information about nuclear sequence variation at hundreds to thousands of loci. Divergent reference capture makes use of molecular data of one species to enrich target loci in other (related) species. This is particularly valuable for nonmodel organisms, for which often no a priori knowledge exists regarding these loci. Here, we have used targeted capture to obtain data for 809 nuclear coding DNA sequences (CDS) in a nonmodel organism, the Eurasian lynx Lynx lynx, using baits designed with the help of the published genome of a related model organism (the domestic cat Felis catus). Using this approach, we were able to survey intraspecific variation at hundreds of nuclear loci in L. lynx across the species? European range. A large set of biallelic candidate SNPs was then evaluated using a high-throughput SNP genotyping platform (Fluidigm), which we then reduced to a final 96 SNP-panel based on assay performance and reliability; validation was carried out with 100 additional Eurasian lynx samples not included in the SNP discovery phase. The 96 SNP-panel developed from CDS performed very successfully in the identification of individuals and in population genetic structure inference (including the assignment of individuals to their source population). In keeping with recent studies, our results show that genic SNPs can be valuable for genetic monitoring of wildlife species.

KW - conservation genetics

KW - single nucleotide polymorphism

KW - hybridization capture

KW - CDS

KW - Eurasian lynx

KW - genetic monitoring

U2 - 10.1111/1755-0998.12924

DO - 10.1111/1755-0998.12924

M3 - Erthygl

VL - 18

SP - 1356

EP - 1373

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

IS - 6

ER -