Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • Makhmadyusuf K Khasimov
    Institute of Basic Biological Problems of Russian Academy of Sciences
  • Ekaterina P Petushkova
    Institute of Basic Biological Problems of Russian Academy of Sciences
  • Anna N Khusnutdinova
    Federal Research Center "Pushchino's center of Biological Research"Institute of Basic Biological Problems of Russian Academy of Sciences
  • Nikolay A Zorin
    Institute of Basic Biological Problems of Russian Academy of Sciences
  • Khorcheska A Batyrova
    Institute of Basic Biological Problems of Russian Academy of Sciences
  • Alexander F Yakunin
    Bangor University
  • Anatoly A Tsygankov
    Institute of Basic Biological Problems of Russian Academy of Sciences

Thiocapsa bogorovii BBS (former name Thiocapsa roseopersicina) contains HydSL hydrogenase belonging to 1e subgroup of NiFe hydrogenases (isp-type). The operon of these hydrogenases contains gene for small subunit (hydS), gene for large subunit (hupL), and genes isp1 and isp2 between them. It is predicted that last two genes code electron transport careers for electron transfer from/to HydSL hydrogenase. However, the interaction between them is unclear. The aim of this study was to determine structural and functional role of T. bogorovii HydS C-terminal end. For this purpose, we modelled all subunits of the complex HydS-HydL-Isp1-Isp2. Hydrophobicity surface analysis of the Isp1 model revealed highly hydrophobic helices suggesting potential membrane localization, as well as the hydrophilic C-terminus, which is likely localized outside of membrane. Isp1 model was docked with models of full length and C-terminal truncated HydSL hydrogenases and results illustrate the possibility of HydSL membrane anchoring via transmembrane Isp1 with essential participation of C-terminal end of HydS in the interaction. C-terminal end of HydS subunit was deleted and our studies revealed that the truncated HydSL hydrogenase detached from cellular membranes in contrast to native hydrogenase. It is known that HydSL hydrogenase in T. bogorovii performs the reaction of elemental sulfur reduction (S0 + H2 = ≥H2S). Cells with truncated HydS produced much less H2S in the presence of H2 and S0. Thus, our data support the conclusion that C-terminal end of HydS subunit participates in interaction of HydSL hydrogenase with Isp1 protein for membrane anchoring and electron transfer.

Allweddeiriau

Iaith wreiddiolSaesneg
Rhif yr erthygl148492
CyfnodolynBiochimica et Biophysica Acta (BBA) - Bioenergetics
Cyfrol1862
Rhif y cyfnodolyn12
Dyddiad ar-lein cynnar4 Medi 2021
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Rhag 2021
Cyhoeddwyd yn allanolIe
Gweld graff cysylltiadau