The parathyroid hormone-responsive B1 gene is interrupted by a t(1;7)(q42;p15) breakpoint associated with Wilms' tumour

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Dangosydd eitem ddigidol (DOI)

  • Ellen Vernon
  • K Malik
    Bristol University
  • P Reynolds
    Bristol University
  • R Powlesland
    Bristol University
  • A Dallosso
    North Bristol NHS Trust
  • S. Jackson
    Bristol University
  • K Henthorn
    Bristol University
  • Keith W. Brown
    Bristol University
Wilms' tumour (WT) has a diverse and complex molecular aetiology, with several different loci identified by cytogenetic and molecular analyses. One such locus is on chromosome 7p, where cytogenetic abnormalities and loss of heterozygosity (LOH) indicate the presence of a Wilms' tumour suppressor gene. In order to isolate a candidate gene for this locus, we have characterized the breakpoint regions at a novel constitutional chromosome translocation (t(1;7)(q42;p15)), found in a child with WT and skeletal abnormalities. We identified two genes that were interrupted by the translocation: the parathyroid hormone-responsive B1 gene (PTH-B1) at 7p and obscurin at 1q. With no evidence for LOH at 1q42, we focused on the characterization of PTH-B1. We detected novel alternately spliced isoforms of PTH-B1, which were expressed in a wide range of adult and foetal tissues. Importantly, expression of two isoforms were disrupted in the WT of the t(1;7) patient. We also identified an additional splice isoform expressed only in 7p LOH tumours. The disruption of PTH-B1 by the t(1;7), together with aberrant splicing in sporadic WTs, suggests that PTH-B1 is a candidate for the 7p Wilms' tumour suppressor gene.
Iaith wreiddiolSaesneg
Tudalennau (o-i)1371-80
Nifer y tudalennau9
CyfnodolynOncogene
Cyfrol22
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 5 Maw 2003
Gweld graff cysylltiadau