TY - JOUR

T1 - Translin and Trax differentially regulate telomere-associated transcript homeostasis

AU - Gomez Escobar, Natalia

AU - Almobadel, Nasser

AU - Alzahrani, Othman

AU - Feichtinger, Julia

AU - Planells Palop, Vicente

AU - Alshehri, Zafer

AU - Thallinger, Gerhard G.

AU - Wakeman, Jane

AU - Mcfarlane, Ramsay

N1 - Cancer Research Wales; Government of the Kingdom of Saudi Arabia; OMICS Centre Graz grant of the Austrian Ministry for Science, Research and Economy

PY - 2016/5/10

Y1 - 2016/5/10

N2 - Translin and Trax proteins are highly conserved nucleic acid binding proteinsthat have been implicated in RNA regulation in a range of biological processesincluding tRNA processing, RNA interference, microRNA degradation duringoncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts.Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomericregions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstratethat human Translin and Trax also controltelomere-associated transcript levels in human cells in a telomere-specific fashion.

AB - Translin and Trax proteins are highly conserved nucleic acid binding proteinsthat have been implicated in RNA regulation in a range of biological processesincluding tRNA processing, RNA interference, microRNA degradation duringoncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts.Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomericregions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstratethat human Translin and Trax also controltelomere-associated transcript levels in human cells in a telomere-specific fashion.

U2 - 10.18632/oncotarget.9278

DO - 10.18632/oncotarget.9278

M3 - Article

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

ER -